PMID- 22103766
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20131121
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 50
IP  - 3
DP  - 2012 Mar
TI  - Evaluation of the liver protection and toxicity of Da-Huang-Zhe-Chong pill in 
      rats.
PG  - 344-50
LID - 10.3109/13880209.2011.604333 [doi]
AB  - CONTEXT: Da-Huang-Zhe-Chong pill (DHZCP), a classical traditional Chinese 
      formula, consists of 12 crude drugs which have been widely used with significant 
      therapeutic effects. Some drugs in this formula have toxicities that might result 
      in some adverse effects of DHZCP. OBJECTIVE: The liver protection and toxicity of 
      DHZCP were first evaluated against chronic carbon tetrachloride (CCl(4))-induced 
      liver injury in rats. MATERIALS AND METHODS: The rats were treated by 
      intraperitoneal injection of 10% CCl(4) for 12 weeks. At the end of week 4, DHZCP 
      at doses of 44 g/kg (high-dose group) and 22 g/kg (low-dose group) was 
      intragastrically administered to CCl(4)-treated rats, once a day for four weeks. 
      At the end of weeks 8 and 12, the general status of the rats, histopathology of 
      liver, serum alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), 
      alkaline phosphatase (ALP) and total bilirubin (TBIL) levels were observed or 
      determined and recorded. By correspondence analysis (CA) on biochemical markers 
      and liver histopathological score (HS), the "dose-time-response" relationship of 
      DHZCP on the hepatic injury rats was evaluated. RESULTS: The results showed that 
      DHZCP exhibited a significant protective effect on liver injury by reversing the 
      biochemical parameters and histopathological changes. However, this 
      hepatoprotective effect may be weakened, or even be transferred to toxicity with 
      the increase of the administration dose (44 g.kg(-1).d(-1)) and time (more than 2 
      months) of this formula. These results were consistent with the histopathological 
      observation and the serum levels. DISCUSSION AND CONCLUSION: Administration of 
      proper dose and time of DHZCP could well play its hepatoprotective effect and 
      even treat hepatitis, but the safety on liver should be considered when 
      large-dose (44 g.kg(-1).d(-1)) DHZCP is used for long time (more than 2 months). 
      We suggest that the administration dose and time of DHZCP in clinical use should 
      not be increased and prolonged, and simultaneously liver function should be 
      regularly monitored.
FAU - Xing, Xiao-yan
AU  - Xing XY
AD  - China Military Institute of Chinese Materia Medica, 302 Military Hospital of 
      China, Beijing, PR China.
FAU - Zhao, Yan-ling
AU  - Zhao YL
FAU - Jia, Lei
AU  - Jia L
FAU - Kong, Wei-jun
AU  - Kong WJ
FAU - Zhong, Yan-wei
AU  - Zhong YW
FAU - Wang, Jia-bo
AU  - Wang JB
FAU - Zhang, Ping
AU  - Zhang P
FAU - Ren, Hui-ling
AU  - Ren HL
FAU - Xiao, Xiao-he
AU  - Xiao XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111121
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Dahuang Zhechong pill)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Protective Agents)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/administration & dosage/*pharmacology/toxicity
MH  - Female
MH  - Injections, Intraperitoneal
MH  - Liver Diseases/physiopathology/*prevention & control
MH  - Liver Function Tests
MH  - Male
MH  - Protective Agents/administration & dosage/*pharmacology/toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 2011/11/23 06:00
MHDA- 2012/06/01 06:00
CRDT- 2011/11/23 06:00
PHST- 2011/11/23 06:00 [entrez]
PHST- 2011/11/23 06:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
AID - 10.3109/13880209.2011.604333 [doi]
PST - ppublish
SO  - Pharm Biol. 2012 Mar;50(3):344-50. doi: 10.3109/13880209.2011.604333. Epub 2011 
      Nov 21.