PMID- 22107032
OWN - NLM
STAT- MEDLINE
DCOM- 20120814
LR  - 20211021
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 79
IP  - 2
DP  - 2012 Feb
TI  - Use of dried blood spots for the determination of genetic variation of 
      interleukin-10, killer immunoglobulin-like receptor and HLA class I genes.
PG  - 114-22
LID - 10.1111/j.1399-0039.2011.01807.x [doi]
AB  - Optimal methods for using dried blood spots (DBSs) for population genetics-based 
      studies have not been well established. Using DBS stored for 8 years from 21 
      pregnant South African women, we evaluated three methods of gDNA extraction with 
      and without whole-genome amplification (WGA) to characterize immune-related 
      genes: interleukin-10 (IL-10), killer immunoglobulin-like receptors (KIRs) and 
      human leukocyte antigen (HLA) class I. We found that the QIAamp DNA mini kit 
      yielded the highest gDNA quality (P< 0.05; Wilcoxon signed rank test) with 
      sufficient yield for subsequent analyses. In contrast, we found that WGA was not 
      reliable for sequence-specific primer polymerase chain reaction (SSP-PCR) 
      analysis of KIR2DL1, KIR2DS1, KIR2DL5 and KIR2DL3 or high-resolution HLA 
      genotyping using a sequence-based approach. We speculate that unequal template 
      amplification by WGA underrepresents gene repertoires determined by 
      sequence-based approaches.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Ndlovu, B G
AU  - Ndlovu BG
AD  - HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R. 
      Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.
FAU - Danaviah, S
AU  - Danaviah S
FAU - Moodley, E
AU  - Moodley E
FAU - Ghebremichael, M
AU  - Ghebremichael M
FAU - Bland, R
AU  - Bland R
FAU - Viljoen, J
AU  - Viljoen J
FAU - Newell, M-L
AU  - Newell ML
FAU - Ndung'u, T
AU  - Ndung'u T
FAU - Carr, W H
AU  - Carr WH
LA  - eng
GR  - K01 TW007793/TW/FIC NIH HHS/United States
GR  - WT_/Wellcome Trust/United Kingdom
GR  - HHSN267200800001G/DK/NIDDK NIH HHS/United States
GR  - U01 AI068632/AI/NIAID NIH HHS/United States
GR  - U01 AI068616/AI/NIAID NIH HHS/United States
GR  - HHSN267200800001C/HD/NICHD NIH HHS/United States
GR  - AI068632/AI/NIAID NIH HHS/United States
GR  - K01-TW00703-04A1/TW/FIC NIH HHS/United States
GR  - N01-DK-9-001/DK/NIDDK NIH HHS/United States
GR  - UM1 AI068632/AI/NIAID NIH HHS/United States
GR  - U01 AI041110/AI/NIAID NIH HHS/United States
GR  - K01 TW007793-04/TW/FIC NIH HHS/United States
GR  - 1 U01 AI068616/AI/NIAID NIH HHS/United States
GR  - 5 U01 AI41110/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111122
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, KIR)
RN  - 130068-27-8 (Interleukin-10)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - DNA/analysis/genetics
MH  - DNA Fingerprinting/*methods
MH  - *Dried Blood Spot Testing
MH  - Female
MH  - Genetic Variation
MH  - Genotype
MH  - Histocompatibility Antigens Class I/*genetics/immunology
MH  - Humans
MH  - Interleukin-10/*genetics/immunology
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Protein Isoforms/*genetics/immunology
MH  - Receptors, KIR/*genetics/immunology
MH  - Sensitivity and Specificity
PMC - PMC3253194
MID - NIHMS339763
COIS- Conflicts of interest: There are no conflicts of interest.
EDAT- 2011/11/24 06:00
MHDA- 2012/08/15 06:00
PMCR- 2013/02/01
CRDT- 2011/11/24 06:00
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2012/08/15 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - 10.1111/j.1399-0039.2011.01807.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Feb;79(2):114-22. doi: 10.1111/j.1399-0039.2011.01807.x. 
      Epub 2011 Nov 22.