PMID- 22108054
OWN - NLM
STAT- MEDLINE
DCOM- 20120221
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 416
IP  - 3-4
DP  - 2011 Dec 16
TI  - The lectin ArtinM binds to mast cells inducing cell activation and mediator 
      release.
PG  - 318-24
LID - 10.1016/j.bbrc.2011.11.033 [doi]
AB  - Mast cells are inflammatory cells that respond to signals of innate and adaptive 
      immunity with immediate and delayed release of mediators. ArtinM, a lectin from 
      Artocarpus integrifolia with immunomodulatory activities, is able to induce mast 
      cell activation, but the mechanisms remain unknown. This study sought to further 
      investigate the effects of the lectin on mast cells. We showed that ArtinM binds 
      to mast cells, possibly to the high affinity receptor for immunoglobulin E (IgE) 
      - FcepsilonRI - and/or to IgE bound to FcepsilonRI. Binding of the lectin resulted in protein 
      tyrosine phosphorylation and release of the pre- and newly-formed mediators, 
      beta-hexosaminidase and LTB(4) by mast cells, activities that were potentiated in 
      the presence of IgE. ArtinM also induced the activation of the transcription 
      factors NFkappaB and NFAT, resulting in expression of some of their target genes such 
      as IL-4 and TNF-alpha. In view of the established significance of mast cells in many 
      immunological and inflammatory reactions, a better understanding of the 
      mechanisms involved in mast cell activation by ArtinM is crucial to the 
      pharmacological application of the lectin.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Barbosa-Lorenzi, Valeria Cintra
AU  - Barbosa-Lorenzi VC
AD  - Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos, FMRP/USP, 
      Ribeirao Preto, SP, Brazil.
FAU - Buranello, Patricia Andressa de Almeida
AU  - Buranello PA
FAU - Roque-Barreira, Maria Cristina
AU  - Roque-Barreira MC
FAU - Jamur, Maria Celia
AU  - Jamur MC
FAU - Oliver, Constance
AU  - Oliver C
FAU - Pereira-da-Silva, Gabriela
AU  - Pereira-da-Silva G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111115
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (NF-kappa B)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Plant Lectins)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Animals
MH  - Artocarpus/*chemistry
MH  - Cell Line
MH  - Gene Expression
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/drug effects/*immunology
MH  - NF-kappa B/agonists
MH  - NFATC Transcription Factors/agonists
MH  - Phosphorylation
MH  - Plant Lectins/*immunology/metabolism/pharmacology
MH  - Rats
MH  - Receptors, IgE/*immunology
MH  - Tyrosine/metabolism
EDAT- 2011/11/24 06:00
MHDA- 2012/02/22 06:00
CRDT- 2011/11/24 06:00
PHST- 2011/11/04 00:00 [received]
PHST- 2011/11/06 00:00 [accepted]
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2012/02/22 06:00 [medline]
AID - S0006-291X(11)02032-8 [pii]
AID - 10.1016/j.bbrc.2011.11.033 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Dec 16;416(3-4):318-24. doi: 
      10.1016/j.bbrc.2011.11.033. Epub 2011 Nov 15.