PMID- 22108150
OWN - NLM
STAT- MEDLINE
DCOM- 20120312
LR  - 20131121
IS  - 1933-2874 (Print)
IS  - 1876-4789 (Linking)
VI  - 5
IP  - 6
DP  - 2011 Nov-Dec
TI  - Relationships between cholesterol efflux and high-density lipoprotein particles 
      in patients with type 2 diabetes mellitus.
PG  - 467-73
LID - 10.1016/j.jacl.2011.06.016 [doi]
AB  - BACKGROUND: High-density lipoprotein (HDL) encompasses a heterogeneous population 
      of lipoproteins with differences in functionality. The impact of HDL 
      heterogeneity on its ability to support HDL-mediated cholesterol efflux has not 
      been previously studied in patients with type 2 diabetes mellitus (T2DM). 
      OBJECTIVES: To examine the relationships between various HDL subtypes and 
      cholesterol efflux from macrophages in patients with T2DM. METHODS: Lipoprotein 
      molecular profiles of 44 patients were studied by NMR spectroscopy. Cholesterol 
      efflux was expressed as percentage efflux of radioactivity from lipid-laden THP-1 
      macrophages preincubated with (3)H-cholesterol and then incubated with serum 
      depleted of apolipoprotein B to provide an HDL-enriched acceptor medium. RESULTS: 
      There was a predominance of small HDL particles (59%) and small putatively 
      atherogenic low-density lipoprotein particles (56%). Neither HDL-C nor ApoA-I 
      concentrations showed statistically significant correlations with percentage 
      cholesterol efflux, but a significant positive relationship was found with the 
      total HDL particle concentration (r = 0.41, P = .005) contributed to largely by 
      medium HDL particles (r = 0.41, P = .006). The correlation between medium-sized 
      HDL particle concentration remained significantly associated with cholesterol 
      efflux when assessed with the use of a linear regression model that included all 
      the HDL lipoprotein subclass concentrations as well as apolipoprotein A-I. 
      Importantly, no statistically significant association was observed between the 
      number of small HDL particles and cholesterol efflux. Hemoglobin A1c showed a 
      significant inverse correlation with cholesterol efflux (r = -0.31, P = .04). 
      CONCLUSION: In patients with moderately controlled type 2 diabetes mellitus, 
      cholesterol efflux from macrophages incubated with apolipoprotein B-depleted 
      plasmas correlated significantly and positively with the concentration of total 
      and medium-sized HDL and not with that of the smallest particles.
CI  - Copyright (c) 2011 National Lipid Association. All rights reserved.
FAU - Tan, Hong Chang
AU  - Tan HC
AD  - Department of Endocrinology, Singapore General Hospital, Outram Road, Singapore 
      169608. tan.hong.chang@singhealth.com.sg
FAU - Tai, E Shyong
AU  - Tai ES
FAU - Sviridov, Dmitri
AU  - Sviridov D
FAU - Nestel, Paul J
AU  - Nestel PJ
FAU - Ng, Cindy
AU  - Ng C
FAU - Chan, Edmund
AU  - Chan E
FAU - Teo, Yvonne
AU  - Teo Y
FAU - Wai, Daniel Chun Hang
AU  - Wai DC
LA  - eng
PT  - Journal Article
DEP - 20110628
PL  - United States
TA  - J Clin Lipidol
JT  - Journal of clinical lipidology
JID - 101300157
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Culture Media)
RN  - 0 (Lipoproteins, HDL)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anthropometry
MH  - Apolipoprotein A-I/blood
MH  - Apolipoproteins B/metabolism
MH  - Biological Transport
MH  - Cell Line, Tumor
MH  - Cholesterol/*blood
MH  - Culture Media/metabolism
MH  - Diabetes Mellitus, Type 2/*blood/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Linear Models
MH  - Lipoproteins, HDL/*blood
MH  - Macrophages/metabolism
MH  - Magnetic Resonance Spectroscopy
MH  - Male
MH  - Middle Aged
EDAT- 2011/11/24 06:00
MHDA- 2012/03/13 06:00
CRDT- 2011/11/24 06:00
PHST- 2011/01/21 00:00 [received]
PHST- 2011/06/20 00:00 [revised]
PHST- 2011/06/21 00:00 [accepted]
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2012/03/13 06:00 [medline]
AID - S1933-2874(11)00623-4 [pii]
AID - 10.1016/j.jacl.2011.06.016 [doi]
PST - ppublish
SO  - J Clin Lipidol. 2011 Nov-Dec;5(6):467-73. doi: 10.1016/j.jacl.2011.06.016. Epub 
      2011 Jun 28.