PMID- 22110210
OWN - NLM
STAT- MEDLINE
DCOM- 20120207
LR  - 20111123
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 31
IP  - 11
DP  - 2011 Nov
TI  - Down-regulation of MiR-206 promotes proliferation and invasion of laryngeal 
      cancer by regulating VEGF expression.
PG  - 3859-63
AB  - MicroRNAs (miRNA) are a class of small noncoding RNAs that regulate the 
      expression of their target genes. The aim of the present study was to explore the 
      effects of miR-206 on laryngeal suamous cell carcinoma (LSCC) cells. The 
      expression level of miR-206 was quantified by qRT-PCR in primary LSCC tissues and 
      corresponding adjacent non-neoplastic tissues. MTT, Matrigel invasion assays and 
      flow cytometry methods were used to test the proliferation, invasion and 
      apoptosis of MiR-206 transfection LSCC cells and a mouse model was used to 
      investigate tumorigenesis. MiR-206 was significantly down regulated in the LSCC 
      tissues. Inverse correlation of miR-206 expression was found with the T grade, 
      nodal metastasis and clinical stage of LSCC. Cell proliferation, migration, 
      invasion and tumorigenesis in the LSCC cells were significantly inhibited and 
      apoptotic cells were also increased after miR-206 tansfection. Furthermore, 
      miR-206 transfection down-regulated the expression of vascular endothelial growth 
      factor (VEGF) in the LSCC cells. The loss of miR-206 may play an important role 
      in the progress of LSCC and miR-206 may function as a novel tumor suppressed 
      miRNA.
FAU - Zhang, Tianhong
AU  - Zhang T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital, Harbin Medical University, Harbin 150081, PR China.
FAU - Liu, Ming
AU  - Liu M
FAU - Wang, Chunrui
AU  - Wang C
FAU - Lin, Chao
AU  - Lin C
FAU - Sun, Yanan
AU  - Sun Y
FAU - Jin, Dejun
AU  - Jin D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn206 microRNA, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, mouse)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Adhesion
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Down-Regulation
MH  - Female
MH  - Flow Cytometry
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*physiology
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Vascular Endothelial Growth Factor A/*antagonists & 
      inhibitors/genetics/metabolism
EDAT- 2011/11/24 06:00
MHDA- 2012/02/09 06:00
CRDT- 2011/11/24 06:00
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2012/02/09 06:00 [medline]
AID - 31/11/3859 [pii]
PST - ppublish
SO  - Anticancer Res. 2011 Nov;31(11):3859-63.