PMID- 22110531
OWN - NLM
STAT- MEDLINE
DCOM- 20120322
LR  - 20240323
IS  - 1740-2530 (Electronic)
IS  - 1740-2522 (Print)
IS  - 1740-2522 (Linking)
VI  - 2012
DP  - 2012
TI  - Enhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid 
      arthritis.
PG  - 295081
LID - 10.1155/2012/295081 [doi]
LID - 295081
AB  - Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 
      cells, but what about the effect of high-mobility group box chromosomal protein 1 
      (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA 
      remains unknown. In the present study, we investigated the mRNA levels of HMGB1, 
      RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with 
      rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the 
      concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And 
      then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. 
      Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients 
      were higher than that in health control (P < 0.05), especially in active RA 
      patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also 
      higher than that in health control (P < 0.05); there was a positive correlation 
      between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in 
      plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL 
      rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 
      cells activation in RA patients.
FAU - Shi, Yan
AU  - Shi Y
AD  - Department of Immunology, Institute of Laboratory Medicine, Jiangsu University, 
      Xuefu Road 301, Zhenjiang 212013, China.
FAU - Sandoghchian Shotorbani, Siamak
AU  - Sandoghchian Shotorbani S
FAU - Su, Zhaoliang
AU  - Su Z
FAU - Liu, Yanfang
AU  - Liu Y
FAU - Tong, Jia
AU  - Tong J
FAU - Zheng, Dong
AU  - Zheng D
FAU - Chen, Jianguo
AU  - Chen J
FAU - Liu, Yingzhao
AU  - Liu Y
FAU - Xu, Yan
AU  - Xu Y
FAU - Jiao, Zhijun
AU  - Jiao Z
FAU - Wang, Shengjun
AU  - Wang S
FAU - Lu, Liwei
AU  - Lu L
FAU - Huang, Xinxiang
AU  - Huang X
FAU - Xu, Huaxi
AU  - Xu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111026
PL  - Egypt
TA  - Clin Dev Immunol
JT  - Clinical & developmental immunology
JID - 101183692
RN  - 0 (Cytokines)
RN  - 0 (HMGB1 Protein)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Arthritis, Rheumatoid/*genetics/*immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cytokines/blood/immunology
MH  - Female
MH  - HMGB1 Protein/*genetics/metabolism
MH  - Humans
MH  - Lymphocyte Activation/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Middle Aged
MH  - RNA, Messenger/analysis
MH  - Th17 Cells/*immunology
PMC - PMC3205666
EDAT- 2011/11/24 06:00
MHDA- 2012/03/23 06:00
PMCR- 2011/10/26
CRDT- 2011/11/24 06:00
PHST- 2011/05/03 00:00 [received]
PHST- 2011/07/05 00:00 [revised]
PHST- 2011/07/08 00:00 [accepted]
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2012/03/23 06:00 [medline]
PHST- 2011/10/26 00:00 [pmc-release]
AID - 10.1155/2012/295081 [doi]
PST - ppublish
SO  - Clin Dev Immunol. 2012;2012:295081. doi: 10.1155/2012/295081. Epub 2011 Oct 26.