PMID- 22111069
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111123
LR  - 20240109
IS  - 2233-7393 (Electronic)
IS  - 2233-7385 (Print)
IS  - 2233-7393 (Linking)
VI  - 47
IP  - 2
DP  - 2011 Aug
TI  - Theaflavin Inhibits LPS-Induced IL-6, MCP-1, and ICAM-1 Expression in Bone 
      Marrow-Derived Macrophages Through the Blockade of NF-kappaB and MAPK Signaling 
      Pathways.
PG  - 104-10
LID - 10.4068/cmj.2011.47.2.104 [doi]
AB  - Theaflavin, the main polyphenol in black tea, has anti-inflammatory, 
      antioxidative, anti-mutagenic, and anti-carcinogenic properties. The aim of this 
      study was to evaluate the effects of theaflavin on lipopolysaccharide 
      (LPS)-induced innate signaling and expression of pro-inflammatory mediators in 
      bone marrow-derived macrophages isolated from ICR mice. The effects of theaflavin 
      on the expression of proinflammatory mediators, LPS-induced nuclear factor-kappa 
      B (NF-kappaB), and mitogen-activated protein kinase (MAPK) signaling pathways were 
      examined by reverse transcriptase-polymerase chain reaction (RT-PCR), Western 
      blotting, and immunofluorescence. LPS-induced interleukin-6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) 
      expression was inhibited by theaflavin. LPS-induced inhibitor kappa B alpha 
      (IkappaBalpha) degradation and nuclear translocation of RelA were blocked by theaflavin. 
      LPS-induced phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2), 
      c-Jun-N-terminal kinase (JNK), and p38 MAPK was inhibited by theaflavin. The 
      inhibitory effect of theaflavin on IL-6, MCP-1, and ICAM-1 expression was 
      completely inhibited by Bay11-7082 (NF-kappaB inhibitor). The inhibitory effect of 
      theaflavin on IL-6 and ICAM-1 expression was inhibited by SB203580 (p38 MAPK 
      inhibitor). The inhibitory effect of theaflavin on MCP-1 expression was inhibited 
      by SP600125 (JNK inhibitor). These results indicate that theaflavin prevents 
      LPS-induced IL-6, MCP-1, and ICAM-1 expression through blockade of NF-kappaB and MAPK 
      signaling pathways in bone marrow-derived macrophages.
FAU - Kim, Seewan
AU  - Kim S
AD  - Korean Minjok Leadership Academy, Hoengseong, Korea.
FAU - Joo, Young-Eun
AU  - Joo YE
LA  - eng
PT  - Journal Article
DEP - 20110831
PL  - Korea (South)
TA  - Chonnam Med J
JT  - Chonnam medical journal
JID - 101564659
PMC - PMC3214874
OTO - NOTNLM
OT  - MAP kinase
OT  - Macrophage
OT  - NF-kappa B
OT  - Theaflavin
EDAT- 2011/11/24 06:00
MHDA- 2011/11/24 06:01
PMCR- 2011/08/01
CRDT- 2011/11/24 06:00
PHST- 2011/05/04 00:00 [received]
PHST- 2011/05/30 00:00 [accepted]
PHST- 2011/11/24 06:00 [entrez]
PHST- 2011/11/24 06:00 [pubmed]
PHST- 2011/11/24 06:01 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - 10.4068/cmj.2011.47.2.104 [doi]
PST - ppublish
SO  - Chonnam Med J. 2011 Aug;47(2):104-10. doi: 10.4068/cmj.2011.47.2.104. Epub 2011 
      Aug 31.