PMID- 22113165
OWN - NLM
STAT- MEDLINE
DCOM- 20120424
LR  - 20191210
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 60
DP  - 2012 Feb 23
TI  - Development and validation of LC/ESI-MS method for the detection and 
      quantification of exogenous ceramide NP in stratum corneum and other layers of 
      the skin.
PG  - 7-13
LID - 10.1016/j.jpba.2011.10.032 [doi]
AB  - Ceramides (CERs) are integral parts of the intercellular lipid lamellae of the 
      stratum corneum (SC), which provide the barrier function of the skin. 
      Administration of CERs deep into the SC may help to restore the barrier function 
      in affected or aged skin. However, quantification of the amount of CER penetrated 
      into the target site needs a selective and sensitive analytical method. 
      Therefore, an LC/ESI-MS method was developed and validated for the detection and 
      quantification of exogenous CER [NP] in the SC as well as other skin layers. The 
      strategy involved synthesis of ceramide [NP]-D3-18 (deuterated CER [NP]) to 
      distinguish it from the endogenous CER [NP] in MS on weight basis. The method was 
      linear over 10-800 ng/ml and sensitive with a limit of detection (LOD) and limit 
      of quantification (LOQ) of 3 and 10 ng/ml, respectively. It was also accurate 
      with within-run and between-run percentage recoveries of 97.1-103.2 and 
      99.0-104.9, respectively. The within-run and between-run relative standard 
      deviations (RSDs) were 0.9-5.4% and 2.1-7.4%, respectively, suggesting the method 
      is precise. The method was highly selective and the matrix effect was too minimal 
      with matrix factor (MF) mean and RSD values of 1.002 and 4.57%, respectively.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Sahle, Fitsum F
AU  - Sahle FF
AD  - Department of Pharmaceutical Technology and Biopharmaceutics, Institute of 
      Pharmacy, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck Str. 4, 
      D-06120 Halle (Saale), Germany.
FAU - Lange, Stefan
AU  - Lange S
FAU - Dobner, Bodo
AU  - Dobner B
FAU - Wohlrab, Johannes
AU  - Wohlrab J
FAU - Neubert, Reinhard H H
AU  - Neubert RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20111106
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Ceramides)
RN  - AR09D82C7G (Deuterium)
SB  - IM
MH  - Administration, Cutaneous
MH  - Ceramides/administration & dosage/*analysis/chemistry
MH  - Chromatography, Liquid/*methods/statistics & numerical data
MH  - Deuterium/chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Skin/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods/statistics & numerical data
EDAT- 2011/11/25 06:00
MHDA- 2012/04/25 06:00
CRDT- 2011/11/25 06:00
PHST- 2011/07/25 00:00 [received]
PHST- 2011/10/27 00:00 [revised]
PHST- 2011/10/28 00:00 [accepted]
PHST- 2011/11/25 06:00 [entrez]
PHST- 2011/11/25 06:00 [pubmed]
PHST- 2012/04/25 06:00 [medline]
AID - S0731-7085(11)00624-8 [pii]
AID - 10.1016/j.jpba.2011.10.032 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2012 Feb 23;60:7-13. doi: 10.1016/j.jpba.2011.10.032. Epub 
      2011 Nov 6.