PMID- 22114334
OWN - NLM
STAT- MEDLINE
DCOM- 20120409
LR  - 20231115
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 3
DP  - 2012 Feb
TI  - Small RNA deep sequencing identifies microRNAs and other small noncoding RNAs 
      from human herpesvirus 6B.
PG  - 1638-49
LID - 10.1128/JVI.05911-11 [doi]
AB  - Roseolovirus, or human herpesvirus 6 (HHV-6), is a ubiquitous human pathogen 
      infecting over 95% of the population by the age of 2 years. As with other 
      herpesviruses, reactivation of HHV-6 can present with severe complications in 
      immunocompromised individuals. Recent studies have highlighted the importance of 
      herpesvirus-derived microRNAs (miRNAs) in modulating both cellular and viral gene 
      expression. An initial report which computed the likelihood of various viruses to 
      encode miRNAs did not predict HHV-6 miRNAs. To experimentally screen for small 
      HHV-6-encoded RNAs, we conducted large-scale sequencing of Sup-T-1 cells 
      lytically infected with a laboratory strain of HHV-6B. This revealed an abundant, 
      60- to 65-nucleotide RNA of unknown function derived from the lytic origin of 
      replication (OriLyt) that gave rise to smaller RNA species of 18 or 19 
      nucleotides. In addition, we identified four pre-miRNAs whose mature forms 
      accumulated in Argonaute 2. In contrast to the case for other betaherpesviruses, 
      HHV-6B miRNAs are expressed from direct repeat regions (DR(L) and DR(R)) located 
      at either side of the genome. All miRNAs are conserved in the closely related 
      HHV-6A variant, and one of them is a seed ortholog of the human miRNA miR-582-5p. 
      Similar to alphaherpesvirus miRNAs, they are expressed in antisense orientation 
      relative to immediate-early open reading frames (ORFs) and thus have the 
      potential to regulate key viral genes.
FAU - Tuddenham, Lee
AU  - Tuddenham L
AD  - Architecture et Reactivite de l'ARN, Universite de Strasbourg, Institut de 
      Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France.
FAU - Jung, Jette S
AU  - Jung JS
FAU - Chane-Woon-Ming, Beatrice
AU  - Chane-Woon-Ming B
FAU - Dolken, Lars
AU  - Dolken L
FAU - Pfeffer, Sebastien
AU  - Pfeffer S
LA  - eng
GR  - 260767/ERC_/European Research Council/International
GR  - G1002523/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111123
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Untranslated)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers
MH  - Fluorescent Antibody Technique
MH  - Genes, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Likelihood Functions
MH  - MicroRNAs/*genetics
MH  - RNA, Untranslated/*genetics
MH  - Replication Origin
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3264354
EDAT- 2011/11/25 06:00
MHDA- 2012/04/10 06:00
PMCR- 2012/08/01
CRDT- 2011/11/25 06:00
PHST- 2011/11/25 06:00 [entrez]
PHST- 2011/11/25 06:00 [pubmed]
PHST- 2012/04/10 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - JVI.05911-11 [pii]
AID - 5911-11 [pii]
AID - 10.1128/JVI.05911-11 [doi]
PST - ppublish
SO  - J Virol. 2012 Feb;86(3):1638-49. doi: 10.1128/JVI.05911-11. Epub 2011 Nov 23.