PMID- 22114714
OWN - NLM
STAT- MEDLINE
DCOM- 20120503
LR  - 20240216
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 11
DP  - 2011
TI  - Tension-compression loading with chemical stimulation results in additive 
      increases to functional properties of anatomic meniscal constructs.
PG  - e27857
LID - 10.1371/journal.pone.0027857 [doi]
LID - e27857
AB  - OBJECTIVE: This study aimed to improve the functional properties of 
      anatomically-shaped meniscus constructs through simultaneous tension and 
      compression mechanical stimulation in conjunction with chemical stimulation. 
      METHODS: Scaffoldless meniscal constructs were subjected to simultaneous tension 
      and compressive stimulation and chemical stimulation. The temporal aspect of 
      mechanical loading was studied by employing two separate five day stimulation 
      periods. Chemical stimulation consisted of the application of a catabolic 
      GAG-depleting enzyme, chondroitinase ABC (C-ABC), and an anabolic growth factor, 
      TGF-beta1. Mechanical and chemical stimulation combinations were studied through a 
      full-factorial experimental design and assessed for histological, biochemical, 
      and biomechanical properties following 4 wks of culture. RESULTS: Mechanical 
      loading applied from days 10-14 resulted in significant increases in compressive, 
      tensile, and biochemical properties of meniscal constructs. When mechanical and 
      chemical stimuli were combined significant additive increases in collagen per wet 
      weight (4-fold), compressive instantaneous (3-fold) and relaxation (2-fold) 
      moduli, and tensile moduli in the circumferential (4-fold) and radial (6-fold) 
      directions were obtained. CONCLUSIONS: This study demonstrates that a stimulation 
      regimen of simultaneous tension and compression mechanical stimulation, C-ABC, 
      and TGF-beta1 is able to create anatomic meniscus constructs replicating the 
      compressive mechanical properties, and collagen and GAG content of native tissue. 
      In addition, this study significantly advances meniscus tissue engineering by 
      being the first to apply simultaneous tension and compression mechanical 
      stimulation and observe enhancement of tensile and compressive properties 
      following mechanical stimulation.
FAU - Huey, Daniel J
AU  - Huey DJ
AD  - Department of Biomedical Engineering, University of California Davis, Davis, 
      California, United States of America.
FAU - Athanasiou, Kyriacos A
AU  - Athanasiou KA
LA  - eng
GR  - R01 AR047839/AR/NIAMS NIH HHS/United States
GR  - R01-AR-047839/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111116
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9007-34-5 (Collagen)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Collagen/metabolism
MH  - *Compressive Strength
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoenzyme Techniques
MH  - Menisci, Tibial/*drug effects/physiology
MH  - Stimulation, Chemical
MH  - *Tensile Strength
MH  - Tissue Engineering
MH  - Transforming Growth Factor beta1/*pharmacology
PMC - PMC3218070
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/11/25 06:00
MHDA- 2012/05/04 06:00
PMCR- 2011/11/16
CRDT- 2011/11/25 06:00
PHST- 2011/06/15 00:00 [received]
PHST- 2011/10/26 00:00 [accepted]
PHST- 2011/11/25 06:00 [entrez]
PHST- 2011/11/25 06:00 [pubmed]
PHST- 2012/05/04 06:00 [medline]
PHST- 2011/11/16 00:00 [pmc-release]
AID - PONE-D-11-10874 [pii]
AID - 10.1371/journal.pone.0027857 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(11):e27857. doi: 10.1371/journal.pone.0027857. Epub 2011 Nov 16.