PMID- 2211842
OWN - NLM
STAT- MEDLINE
DCOM- 19901121
LR  - 20141120
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 145
IP  - 1
DP  - 1990 Oct
TI  - Bumetanide-sensitive Na+/K+/Cl- transporter is stimulated by phorbol ester and 
      different mitogens in quiescent human skin fibroblasts.
PG  - 30-8
AB  - In this study we investigated the correlation between the mitogenic effect and 
      stimulation of Rb+ (K+) fluxes in human skin fibroblasts treated by purified 
      growth factors. Both K+ transporters, bumetanide-sensitive and ouabain-sensitive, 
      are stimulated 2-3-fold after addition of either fetal calf serum or purified 
      recombinant growth factors to quiescent G0/G1 human skin fibroblasts. Three 
      groups of mitogens were compared: i) the phorbol ester 
      2-O-tetradecanoyl-phorbol-13-acetate (TPA); ii) growth factors that stimulate 
      inositol phosphate hydrolysis and subsequently activate protein kinase 
      C--fibroblast growth factor (FGF), platelet derived growth factor (PDGF), and 
      alpha-thrombin; and iii) growth factors that do not activate kinase 
      C--insulin-like growth factor-1 (IGF-1), and transforming like 
      growth-factor-alpha (TGF-alpha). The three groups of mitogens stimulated human 
      skin fibroblasts proliferation and Rb+ influxes in a similar dose-dependent 
      fashion. The results indicate that both the bumetanide-sensitive and the 
      ouabain-sensitive Rb+ fluxes are stimulated by protein kinase C-dependent and by 
      the protein kinase C-independent pathways of the mitogenic signal.
FAU - Panet, R
AU  - Panet R
AD  - Department of Medical Biophysics and Nuclear Medicine, Hadassah University 
      Hospital, Jerusalem, Israel.
FAU - Atlan, H
AU  - Atlan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Carrier Proteins)
RN  - 0 (Chlorides)
RN  - 0 (Growth Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitogens)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.4.21.5 (Thrombin)
RN  - MLT4718TJW (Rubidium)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Bumetanide/pharmacology
MH  - Carrier Proteins/*drug effects
MH  - Cell Division/physiology
MH  - Chlorides/metabolism
MH  - DNA Replication
MH  - Fibroblast Growth Factors/physiology
MH  - Fibroblasts/metabolism
MH  - Growth Substances/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Insulin-Like Growth Factor I/physiology
MH  - Membrane Proteins/*drug effects
MH  - Mitogens/*pharmacology
MH  - Platelet-Derived Growth Factor/physiology
MH  - Potassium/metabolism
MH  - Protein Kinase C/physiology
MH  - Rubidium/metabolism
MH  - Skin/cytology/metabolism
MH  - Sodium/metabolism
MH  - Sodium-Potassium-Chloride Symporters
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Thrombin/physiology
MH  - Transforming Growth Factor alpha/physiology
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1002/jcp.1041450106 [doi]
PST - ppublish
SO  - J Cell Physiol. 1990 Oct;145(1):30-8. doi: 10.1002/jcp.1041450106.