PMID- 22118815
OWN - NLM
STAT- MEDLINE
DCOM- 20120706
LR  - 20181201
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 78
DP  - 2012 Apr
TI  - Histopathological, histomorphometrical, and immunohistochemical biomarkers in 
      flounder (Platichthys flesus) from the southern Baltic Sea.
PG  - 14-21
LID - 10.1016/j.ecoenv.2011.10.025 [doi]
AB  - Flounder (Platichthys flesus), collected in late fall of 2009 from four coastal 
      sites in the southern Baltic Sea including the Gulf of Gdansk (GoG), were 
      investigated for a suite of biomarkers of contaminant effects. The biomarkers 
      included liver histopathologies, which were diagnosed and assessed using commonly 
      applied lesion categories, the size and density of melano-macrophage aggregates 
      (MMAs), expression of proliferating cell nuclear antigen (PCNA) and of cytochrome 
      P450 1A (CYP1A) in liver as well as the size and density of MMAs and density of 
      Perls'-positive cells in the spleen. The prevalence of liver lesions differed 
      among the sites. Most frequently occurring were non-specific and early 
      toxicopathic non-neoplastic lesions. Mean MMA size was in the range of 264-519 
      mum(2) and 717-2137 mum(2) in liver and spleen, respectively, and density was in 
      the range of 6-13 and 15-26 MMAmm(-2), respectively. Mean density of 
      PCNA-positive hepatocytes was in the range of 300-1281 cellsmm(-2). These 
      histomorphometrical biomarkers correlated positively with the muscle Hg, Sigma(7)PCB, 
      and SigmaDDT residues and negatively with the indices of general liver condition. 
      They showed significant differences between the sites, which were in line with 
      the spatial prevalence of liver lesions and CYP1A induction. Overall, the 
      biomarker responses were more pronounced in the GoG sites in comparison to those 
      outside the Gulf, which confirms some earlier results and broadens the knowledge 
      of contaminant effects in the Polish coastal zone of the Baltic Sea.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Dabrowska, Henryka
AU  - Dabrowska H
AD  - National Marine Fisheries Research Institute, Department of Food and 
      Environmental Chemistry, KoLLataja 1, 81-332 Gdynia, Poland. 
      henryka.dabrowska@mir.gdynia.pl
FAU - Ostaszewska, Teresa
AU  - Ostaszewska T
FAU - Kamaszewski, Maciej
AU  - Kamaszewski M
FAU - Antoniak, Agnieszka
AU  - Antoniak A
FAU - Napora-Rutkowski, Lukasz
AU  - Napora-Rutkowski L
FAU - Kopko, Orest
AU  - Kopko O
FAU - Lang, Thomas
AU  - Lang T
FAU - Fricke, Nicolai F
AU  - Fricke NF
FAU - Lehtonen, Kari K
AU  - Lehtonen KK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111125
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Biomarkers)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Baltic States
MH  - Biomarkers/metabolism
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Environmental Monitoring/*methods
MH  - Female
MH  - Flounder/*metabolism/physiology
MH  - Hepatocytes/metabolism/pathology
MH  - Liver/drug effects/metabolism/pathology
MH  - Muscles/metabolism
MH  - Oceans and Seas
MH  - Polychlorinated Biphenyls/metabolism/toxicity
MH  - Seasons
MH  - Water Pollutants, Chemical/*metabolism/toxicity
EDAT- 2011/11/29 06:00
MHDA- 2012/07/07 06:00
CRDT- 2011/11/29 06:00
PHST- 2011/08/18 00:00 [received]
PHST- 2011/10/19 00:00 [revised]
PHST- 2011/10/25 00:00 [accepted]
PHST- 2011/11/29 06:00 [entrez]
PHST- 2011/11/29 06:00 [pubmed]
PHST- 2012/07/07 06:00 [medline]
AID - S0147-6513(11)00367-8 [pii]
AID - 10.1016/j.ecoenv.2011.10.025 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2012 Apr;78:14-21. doi: 10.1016/j.ecoenv.2011.10.025. 
      Epub 2011 Nov 25.