PMID- 22119900
OWN - NLM
STAT- MEDLINE
DCOM- 20120523
LR  - 20221029
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 163
IP  - 2
DP  - 2012 Feb
TI  - Induction of autophagy enhances porcine reproductive and respiratory syndrome 
      virus replication.
PG  - 650-5
LID - 10.1016/j.virusres.2011.11.008 [doi]
AB  - Autophagy is an evolutionarily conserved lysosome-dependent degradation pathway 
      that acts in the maintenance of cellular homeostasis and plays important 
      functions in viral replication and pathogenesis. In this study, we investigated 
      the role of autophagy in the replication of porcine reproductive and respiratory 
      syndrome virus (PRRSV), an agent that has caused devastating losses in the 
      international swine industry since the late 1980s. Using protein quantification 
      and microscopy, we observed that PRRSV infection results in LC3-I/II conversion, 
      an increased accumulation of punctate GFP-LC3-expressing cells, and a higher 
      number of autophagosome-like double-membrane vesicles in the cytoplasm of host 
      cells. Inhibition of autophagy using 3-methyladenine (3-MA) or small interfering 
      RNAs targeting ATG7 and Beclin-1 led to a significant reduction in PRRSV titers 
      and protein expression. Conversely, induction of autophagy by rapamycin resulted 
      in increased viral replication. These results demonstrate that PRRSV infection 
      induces autophagy which, in turn, enhances viral replication efficiency.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chen, Quangang
AU  - Chen Q
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, PR China.
FAU - Fang, Liurong
AU  - Fang L
FAU - Wang, Dang
AU  - Wang D
FAU - Wang, Shaohua
AU  - Wang S
FAU - Li, Ping
AU  - Li P
FAU - Li, Mao
AU  - Li M
FAU - Luo, Rui
AU  - Luo R
FAU - Chen, Huanchun
AU  - Chen H
FAU - Xiao, Shaobo
AU  - Xiao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111120
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
EIN - Virus Res. 2023 Jan 2;323:198963. PMID: 36308865
MH  - Animals
MH  - *Autophagy
MH  - Cell Line
MH  - Humans
MH  - Macrophages/ultrastructure/virology
MH  - Phagosomes/metabolism/ultrastructure
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity/physiology
MH  - Swine
MH  - *Virus Replication
PMC - PMC7114478
EDAT- 2011/11/29 06:00
MHDA- 2012/05/24 06:00
PMCR- 2011/11/19
CRDT- 2011/11/29 06:00
PHST- 2011/07/25 00:00 [received]
PHST- 2011/11/06 00:00 [revised]
PHST- 2011/11/11 00:00 [accepted]
PHST- 2011/11/29 06:00 [entrez]
PHST- 2011/11/29 06:00 [pubmed]
PHST- 2012/05/24 06:00 [medline]
PHST- 2011/11/19 00:00 [pmc-release]
AID - S0168-1702(11)00457-6 [pii]
AID - 10.1016/j.virusres.2011.11.008 [doi]
PST - ppublish
SO  - Virus Res. 2012 Feb;163(2):650-5. doi: 10.1016/j.virusres.2011.11.008. Epub 2011 
      Nov 20.