PMID- 22121799
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20191210
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 36
IP  - 17
DP  - 2011 Sep
TI  - [Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in 
      Glycyrrhiza uralensis by HPLC-fLD after immunoaffinity column with online 
      post-column photochemical derivatization].
PG  - 2342-6
AB  - OBJECTIVE: To develop a method for the simultaneous determination of aflatoxin 
      B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after 
      immunoaffinity column with online post-column photochemical derivatization. 
      METHOD: Sample was extracted with MeOH: H2O (80:20) and cleaned up by 
      immunoaffinity column. The toxins were separated by reversed-phase HPLC and the 
      mobile phase was consisted of methanol and 0.5% acetic acid solution with 
      gradient elution. The determination was carried out by fluorescence detector 
      after photochemical derivatization. RESULT: The detection limits of aflatoxin G2, 
      G1, B2, B1 and ochratoxin A were 0.02, 0.06, 0.015, 0.03 and 0.25 microg x 
      kg(-1), respectively. The recoveries of analytes were from 76.0% to 103% and the 
      relative standard deviations (RSDs) were below 13%. CONCLUSION: The method is a 
      simple, accurate and can be used to determine the contents of aflatoxin B1, B2, 
      G1, G2 and ochratoxin A in G. uralensis simultaneously.
FAU - Wei, Riwei
AU  - Wei R
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China. weiriweihao@126.com
FAU - Yang, Xiaoli
AU  - Yang X
FAU - Qiu, Feng
AU  - Qiu F
FAU - Yang, Meihua
AU  - Yang M
FAU - Qin, Jieping
AU  - Qin J
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Aflatoxins)
RN  - 0 (Ochratoxins)
RN  - 1779SX6LUY (ochratoxin A)
SB  - IM
MH  - Aflatoxins/*chemistry/isolation & purification
MH  - Chromatography, Affinity/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Glycyrrhiza uralensis/*chemistry
MH  - Ochratoxins/*chemistry/isolation & purification
MH  - Photochemical Processes
EDAT- 2011/11/30 06:00
MHDA- 2011/12/16 06:00
CRDT- 2011/11/30 06:00
PHST- 2011/11/30 06:00 [entrez]
PHST- 2011/11/30 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(17):2342-6.