PMID- 22121953
OWN - NLM
STAT- MEDLINE
DCOM- 20120514
LR  - 20120123
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 39
IP  - 2
DP  - 2012 Feb
TI  - FISH for MYC amplification and anti-MYC immunohistochemistry: useful diagnostic 
      tools in the assessment of secondary angiosarcoma and atypical vascular 
      proliferations.
PG  - 234-42
LID - 10.1111/j.1600-0560.2011.01843.x [doi]
AB  - BACKGROUND: Secondary angiosarcoma and benign but microscopically atypical 
      vascular proliferations (herein referred to as atypical vascular lesion or AVL) 
      are rare consequences of radiation therapy and/or chronic lymphedema most 
      commonly seen in breast cancer patients. Differentiating angiosarcoma from AVL 
      can be difficult due to overlapping clinical and microscopic features. Recently, 
      amplification of MYC has been associated with 55-100% of secondary angiosarcomas 
      but is reportedly absent in AVL. We examined a series of secondary angiosarcoma 
      and AVL for MYC amplification by fluorescence in situ hybridization (FISH) and 
      expression by immunohistochemistry to investigate the diagnostic utility for 
      discriminating angiosarcoma from AVL. METHODS: Cases of secondary angiosarcoma (n 
      = 8) and AVL (n = 4) were retrieved from our archives and examined by FISH and 
      immunohistochemistry. RESULTS: All angiosarcoma cases (8/8 = 100%) demonstrated 
      high-level MYC amplification by FISH, whereas all AVLs (0/4 = 0%) were negative 
      for MYC amplification. Additionally, all angiosarcoma cases (8/8 = 100%) 
      demonstrated nuclear positivity for MYC, whereas all AVLs (0/4 = 0%) were 
      negative. CONCLUSION: Amplification of MYC and nuclear expression of MYC is 
      present in secondary angiosarcoma but not AVL. These ancillary tests can be 
      useful to distinguish angiosarcoma from AVL in difficult cases.
CI  - Copyright (c) 2011 John Wiley & Sons A/S.
FAU - Fernandez, Anthony P
AU  - Fernandez AP
AD  - Department of Dermatology, Cleveland Clinic Foundation, Cleveland, OH, USA.
FAU - Sun, Yang
AU  - Sun Y
FAU - Tubbs, Raymond R
AU  - Tubbs RR
FAU - Goldblum, John R
AU  - Goldblum JR
FAU - Billings, Steven D
AU  - Billings SD
LA  - eng
PT  - Journal Article
DEP - 20111129
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Nucleus/genetics/metabolism/pathology
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Expression Regulation, Neoplastic
MH  - *Hemangiosarcoma/genetics/metabolism/pathology/secondary
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - *Neoplasms, Second Primary
MH  - *Proto-Oncogene Proteins c-myc/genetics/metabolism
MH  - *Vascular Diseases/genetics/metabolism/pathology
EDAT- 2011/11/30 06:00
MHDA- 2012/05/15 06:00
CRDT- 2011/11/30 06:00
PHST- 2011/11/30 06:00 [entrez]
PHST- 2011/11/30 06:00 [pubmed]
PHST- 2012/05/15 06:00 [medline]
AID - 10.1111/j.1600-0560.2011.01843.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 2012 Feb;39(2):234-42. doi: 10.1111/j.1600-0560.2011.01843.x. 
      Epub 2011 Nov 29.