PMID- 22125077
OWN - NLM
STAT- MEDLINE
DCOM- 20120316
LR  - 20211203
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 821
DP  - 2012
TI  - Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors.
PG  - 349-59
LID - 10.1007/978-1-61779-430-8_22 [doi]
AB  - Amplification of the gene encoding the epidermal growth factor receptor (EGFR) 
      occurs commonly in glioblastoma (GBM), leading to activation of downstream 
      kinases, including phosphatidylinositol 3'-kinase (PI3K), Akt, and mammalian 
      target of rapamycin (mTOR). A serine-threonine kinase, mTOR controls cell growth 
      by regulating mRNA translation, metabolism, and autophagy; acting as both a 
      downstream effector and upstream regulator of PI3K. These signaling functions are 
      distributed between at least two distinct complexes, mTORC1 and mTORC2 with 
      respect to pathway specificity. We have investigated mTOR signaling in glioma 
      cells with the allosteric mTORC1 inhibitor rapamycin, the mTORC1/2 inhibitor 
      Ku-0063794, a dual PI3K/mTORC1/2 kinase inhibitor PI-103, and siRNA against 
      raptor, rictor, or mTOR, and evaluated the value of mTOR inhibitors for the 
      treatment of glioblastoma.
FAU - Fan, Qi-Wen
AU  - Fan QW
AD  - Department of Neurology, University of California, San Francisco, CA, USA. 
      qiwen.fan@ucsf.edu
FAU - Weiss, William A
AU  - Weiss WA
LA  - eng
GR  - R01 CA102321/CA/NCI NIH HHS/United States
GR  - R01 CA133091/CA/NCI NIH HHS/United States
GR  - CA128583/CA/NCI NIH HHS/United States
GR  - P01 CA081403/CA/NCI NIH HHS/United States
GR  - CA148699/CA/NCI NIH HHS/United States
GR  - P50 CA097257/CA/NCI NIH HHS/United States
GR  - R01 CA148699/CA/NCI NIH HHS/United States
GR  - CA097257/CA/NCI NIH HHS/United States
GR  - T32 CA128583/CA/NCI NIH HHS/United States
GR  - NS055750/NS/NINDS NIH HHS/United States
GR  - PCA133091/PHS HHS/United States
GR  - R01 NS055750/NS/NINDS NIH HHS/United States
GR  - CA102321/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CRTC2 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Furans)
RN  - 0 (Morpholines)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (PI103)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (Pyridines)
RN  - 0 (Pyrimidines)
RN  - 0 (RICTOR protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RPTOR protein, human)
RN  - 0 (Rapamycin-Insensitive Companion of mTOR Protein)
RN  - 0 (Regulatory-Associated Protein of mTOR)
RN  - 0 (Transcription Factors)
RN  - 81HJG228AB (Ku 0063794)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/antagonists & inhibitors/genetics
MH  - Carrier Proteins/antagonists & inhibitors/genetics
MH  - Cell Line, Tumor
MH  - ErbB Receptors/metabolism
MH  - Furans/pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Glioblastoma/*enzymology
MH  - Humans
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Morpholines/pharmacology
MH  - Multiprotein Complexes
MH  - Oncogene Protein v-akt/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Kinase Inhibitors/*pharmacology
MH  - Proteins/*antagonists & inhibitors
MH  - Pyridines/pharmacology
MH  - Pyrimidines/pharmacology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Rapamycin-Insensitive Companion of mTOR Protein
MH  - Regulatory-Associated Protein of mTOR
MH  - Signal Transduction
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases
MH  - Transcription Factors/*antagonists & inhibitors
PMC - PMC3402221
MID - NIHMS391829
EDAT- 2011/11/30 06:00
MHDA- 2012/03/17 06:00
PMCR- 2013/01/01
CRDT- 2011/11/30 06:00
PHST- 2011/11/30 06:00 [entrez]
PHST- 2011/11/30 06:00 [pubmed]
PHST- 2012/03/17 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.1007/978-1-61779-430-8_22 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;821:349-59. doi: 10.1007/978-1-61779-430-8_22.