PMID- 22126789
OWN - NLM
STAT- MEDLINE
DCOM- 20130506
LR  - 20181201
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 31
IP  - 11
DP  - 2011 Nov
TI  - [Strontium ranelate promotes osteogenic differentiation of rat bone marrow 
      mesenchymal stem cells by increasing bone morphogenetic protein-7 expression].
PG  - 1949-53
AB  - OBJECTIVE: To explore the role of bone morphogenetic protein-7 (BMP-7) in 
      strontium ranelate (Sr)-induced osteogenic differentiation of bone marrow 
      mesenchymal stem cells (BMSCs). METHODS: BMSCs were isolated from 4-week-old rats 
      and cultured in vitro. The third or fourth passages of BMSCs were examined using 
      alkaline phosphatase kit for changes in ALP activity in response to treatment 
      with different concentrations of Sr. Calcium nodules in the induced cells were 
      detected using alizarin red staining, and the cellular BMP-7 expression was 
      detected by Western blotting. RESULTS: Within the concentration range of 0.1-3.0 
      mmol/L, Sr dose-dependently increased ALP activity in rat BMSCs. ALP activity 
      reached the highest level after treatment with 3 mmol/L Sr, which also 
      significantly promoted the formation of calcium nodules. Within the range of 
      0.1-3.0 mmol/L, Sr dose-dependently enhanced the expression of BMP-7, and its 
      peak expression occurred following 3 mmol/L Sr treatment. Noggin (100 ng/ml), an 
      inhibitor of BMP-7, obviously suppressed Sr-induced over-expression of BMP-7 and 
      reduced ALP activity and calcium nodule formation in the BMSCs. CONCLUSION: Sr 
      promotes osteogenic differentiation of rat BMSCs by increasing the expression of 
      BMP-7.
FAU - Li, Zheng
AU  - Li Z
AD  - Southern Medical University, Guangzhou 510515, China. lizheng19850125@163.com
FAU - Wang, Yang
AU  - Wang Y
FAU - Wang, Xiao-na
AU  - Wang XN
FAU - Lan, Ai-ping
AU  - Lan AP
FAU - Wu, Wen
AU  - Wu W
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Bmp7 protein, rat)
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Thiophenes)
RN  - 04NQ160FRU (strontium ranelate)
SB  - IM
MH  - Animals
MH  - Bone Density Conservation Agents/pharmacology
MH  - Bone Marrow Cells/*cytology
MH  - Bone Morphogenetic Protein 7/genetics/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Organometallic Compounds/*pharmacology
MH  - Osteoblasts/*cytology
MH  - Osteogenesis/drug effects
MH  - Rats
MH  - Thiophenes/*pharmacology
EDAT- 2011/12/01 06:00
MHDA- 2013/05/07 06:00
CRDT- 2011/12/01 06:00
PHST- 2011/12/01 06:00 [entrez]
PHST- 2011/12/01 06:00 [pubmed]
PHST- 2013/05/07 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2011 Nov;31(11):1949-53.