PMID- 22127700
OWN - NLM
STAT- MEDLINE
DCOM- 20120127
LR  - 20191210
IS  - 1529-0131 (Electronic)
IS  - 0004-3591 (Linking)
VI  - 63
IP  - 12
DP  - 2011 Dec
TI  - Early diagnosis of arthritis in mice with collagen-induced arthritis, using a 
      fluorogenic matrix metalloproteinase 3-specific polymeric probe.
PG  - 3824-32
LID - 10.1002/art.30628 [doi]
AB  - OBJECTIVE: Early treatment based on an early diagnosis of rheumatoid arthritis 
      (RA) could halt progression of the disease, but early diagnosis is often 
      difficult. Matrix metalloproteinase 3 (MMP-3) is thought to be particularly 
      important in the pathogenesis of RA. The aim of this study was to investigate 
      whether an MMP-3-specific polymeric probe could be used for early diagnosis and 
      for visualizing the progression of arthritis, using a near-infrared fluorescence 
      (NIRF) imaging system. METHODS: The MMP-3-specific polymeric probe was developed 
      by conjugating NIRF dye, MMP substrate peptide, and dark quencher to 
      self-assembled chitosan nanoparticles. One hour after intravenous administration 
      of the probe, fluorescent images of mice with collagen-induced arthritis at 
      different stages of disease development were obtained. The correlation between 
      the fluorescence recovered in in vivo imaging when using an MMP-3-specific 
      polymeric probe and up-regulated MMP-3 activity in the joint tissues was 
      evaluated by Western blotting and immunohistochemical staining. Histologic 
      analysis and micro-computed tomography (micro-CT) were also used to assess 
      arthritis progression. RESULTS: A significantly higher NIRF signal was recovered 
      from arthritic joints compared with normal joints at 14 days after the first 
      immunization, before any erythema or swelling could be observed with the naked 
      eye or any erosion was detected by histologic analysis or micro-CT. The results 
      of immunohistochemical analysis and Western blotting confirmed that the 
      fluorescence recovered in the in vivo imaging was related to up-regulated MMP-3 
      activity in the joint tissues. CONCLUSION: An MMP-3-specific polymeric probe 
      provided clear early diagnosis of arthritis and visualization of arthritis 
      progression using an NIRF imaging system. This approach could be used for early 
      diagnosis and for monitoring drug and surgical therapies in individual cases.
CI  - Copyright (c) 2011 by the American College of Rheumatology.
FAU - Ryu, Ju Hee
AU  - Ryu JH
AD  - Korea Institute of Science and Technology, Seoul, and Seoul National University, 
      Seoul, Republic of Korea.
FAU - Lee, Aeju
AU  - Lee A
FAU - Chu, Jun-Uk
AU  - Chu JU
FAU - Koo, Heebeom
AU  - Koo H
FAU - Ko, Chang-Yong
AU  - Ko CY
FAU - Kim, Han Sung
AU  - Kim HS
FAU - Yoon, Soo-Young
AU  - Yoon SY
FAU - Kim, Byung-Soo
AU  - Kim BS
FAU - Choi, Kuiwon
AU  - Choi K
FAU - Kwon, Ick Chan
AU  - Kwon IC
FAU - Kim, Kwangmeyung
AU  - Kim K
FAU - Youn, Inchan
AU  - Youn I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Biomarkers)
RN  - 0 (Fluorescent Dyes)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*diagnosis/*metabolism/pathology
MH  - Arthrography
MH  - Biomarkers/metabolism
MH  - Diagnostic Imaging/*methods
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - *Early Diagnosis
MH  - Fluorescent Dyes/*metabolism
MH  - Knee Joint/metabolism/pathology
MH  - Male
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Nanoparticles
MH  - Tomography, X-Ray Computed
EDAT- 2011/12/01 06:00
MHDA- 2012/01/28 06:00
CRDT- 2011/12/01 06:00
PHST- 2011/12/01 06:00 [entrez]
PHST- 2011/12/01 06:00 [pubmed]
PHST- 2012/01/28 06:00 [medline]
AID - 10.1002/art.30628 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2011 Dec;63(12):3824-32. doi: 10.1002/art.30628.