PMID- 22128732
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Determination of 12 steroid hormone residues in pig tissues by liquid 
      chromatography-tandem mass spectrometry combining with library search].
PG  - 712-7
AB  - A method was developed for the simultaneous determination and identification of 
      12 steroid hormone residues in pig tissues, including stanolone, aldosterone, 
      boldenone, danazol, metandienone, methyltestosterone, nadrolone, norethindrone, 
      progesterone, stanozolol, testosterone and testosterone propionate, using liquid 
      chromatography-tandem triple-quadrupole linear ion trap mass 
      spectrometry(LC-MS/MS). Homogenized pig tissue samples were purified with a 
      Waters MCX solid phase extraction column after enzymatic hydrolysis by 
      beta-glucuronidase, then separated on a Venusil MP C18 column (100 mm x 2, 1 mm, 
      3 microm) using gradient elution with the mobile phases of acetonitrile and water 
      with 0.1% (v/v) formic acid. A multiple reaction monitoring (MRM) as survey scan 
      and an enhanced product ion (EPI) scan as dependent scan were performed in an 
      information dependent acquisition (IDA) experiment. The compound identification 
      was carried out by library search with a newly developed MS/MS library based on 
      EPI spectra at three different collision energies in positive mode. The results 
      showed that the limits of detection (LODs, S/N = 3) were in the range of 0.2 - 
      0.5 microg/kg for the steroid hormones, and with a good linearity (r > 0.99) 
      ranged from 0.5 to 100.0 microg/L. The average recoveries (n = 6) of the 12 
      steroid hormones spiked in pig tissue samples at 5.0 microg/kg ranged from 72.0% 
      to 98.1% with the relative standard deviations (RSDs) between 3.1% and 12.5%. The 
      method was applied for the qualitative and quantitative determination of steroid 
      hormone residues in pig tissues with sensitive and accurate characteristics.
FAU - Cai, Qinren
AU  - Cai Q
AD  - Technical Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 
      519015, China. mr.cai@vip.163.com
FAU - Feng, Jiawang
AU  - Feng J
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Peng, Yufen
AU  - Peng Y
FAU - Xue, Liangchen
AU  - Xue L
FAU - Du, Zhifeng
AU  - Du Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Hormones)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 4964P6T9RB (Aldosterone)
RN  - V9EFU16ZIF (Methyltestosterone)
SB  - IM
MH  - Aldosterone/analysis
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Dihydrotestosterone/analysis
MH  - Food Analysis/methods
MH  - Food Contamination/*analysis
MH  - Hormones/*analysis
MH  - Methyltestosterone/analysis
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):712-7.