PMID- 22128737
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Determination of polybrominated diphenyl ethers in human serum using solid-phase 
      extraction and gas chromatography coupled with negative chemical ionization mass 
      spectrometry].
PG  - 743-9
AB  - A simplified analytical method comprised of solid-phase extraction (SPE) and gas 
      chromatography coupled with negative chemical ionization mass spectrometry 
      (GC-NCI/MS) has been developed for the determination of 10 polybrominated 
      diphenyl ethers (PBDEs) congeners in human serum. After the extraction by Oasis 
      HLB custom-made SPE cartridges, the lipids in serum were decomposed by 
      concentrated sulfuric acid directly added on the SPE column. The solvent for 
      protein cleanup and the SPE conditions, such as elution solvent and its volume 
      were optimized. The recoveries of the PBDEs spiked in fetal bovine serum relative 
      to internal standards were in the range of 78.5% - 109.7% at five spiked levels 
      (three spiked levels for BDE-209). The intra-day relative standard deviations 
      (RSDs) were between 0.3% and 7.4%, while the inter-day RSDs were between 1.42% 
      and 14.1%. The limits of detection (LOD, S/N = 3) and the limits of 
      quantification (LOQ, S/N = 10) were in the range of 0.10 - 0.27 ng/L and 0.35 
      -0.91 ng/L respectively for all PBDEs, except BDE-209. The LOQ (blank 
      concentration value x 3) for BDE-209 was 7.91 ng/L. The method was verified by 
      accurate analysis of organic contaminant standard reference materials (SRM) 1957 
      and 1958. The results indicated that the proposed method is sensitive, accurate, 
      fast, simple, low solvent consumption and suitable for the determination of tri- 
      to deca-BDE in human serum.
FAU - Huang, Feifei
AU  - Huang F
AD  - National Institute of Nutrition and Food Safety, Chinese Center for Disease 
      Control and Prevention, Beijing 100050, China.
FAU - Zhao, Yunfeng
AU  - Zhao Y
FAU - Li, Jingguang
AU  - Li J
FAU - Wu, Yongning
AU  - Wu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Environmental Pollutants)
RN  - 0 (Halogenated Diphenyl Ethers)
SB  - IM
MH  - Environmental Pollutants/*blood
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Halogenated Diphenyl Ethers/*blood
MH  - Humans
MH  - Solid Phase Extraction/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/methods
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):743-9.