PMID- 22128739
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Simultaneous determination of 9 heterocyclic aromatic amine in poultry products 
      by solid-phase extraction-high performance liquid chromatography].
PG  - 755-61
AB  - A method was developed for the simultaneous determination of 9 heterocyclic 
      aromatic amines (HAAs) including 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 
      2-amino-3,4-dimethylimidazo[4, 5-f] quinoline (MeIQ), 2-amino-3, 
      8-dimethylimidazo [4, 5-f] quinoxaline (MeIQx), 2-amino-3, 4, 8-trimethyl-imidazo 
      [4,5-f quinoxaline (4,8-DiMeIQx), 2-amino-1-methyl-6-phenylimidazo [4,5-b] 
      pyridine (PhIP), 3-amino-1 -methyl-5H-pyrido [4, 3-b] indole (Trp-p-2), 
      3-amino-1, 4-dimethyl-5H-pyrido [4,3-b] indole (Trp-p-1), 9H-pyrido [3,4-b] 
      indole (Norharman), 1-methyl-9H-pyrido 3, 4-b] indole (Harman)) in poultry 
      products using solid-phase extraction-high performance liquid chromatography 
      (SPE-HPLC). The performance of 3 different organic extraction solutions, 2 solid 
      phase extraction (SPE) procedures, three different reversed-phase columns and 
      five different mobile phases were tested for optimizing separation conditions of 
      the 9 HAAs from poultry products. In the end, ethyl acetate was selected as the 
      extraction solution, and the extract was purified with propylsulfonic acid silica 
      (PRS) and C18 SPE columns. The analysis was performed on a TSK-gel ODS-80TM 
      column using a gradient elution with the mobile phases of 0.05 mol/L aqueous 
      acetic acid-ammonium acetate buffer (pH 3.4) and acetonitrile. The results showed 
      that the average recoveries (n = 6) of the 9 HAAs spiked in meat samples at 3 
      levels ranged from 60.47% to 90.55% with the relative standard deviations (RSDs) 
      between 0.49% and 9.74%, and the limits of detection (LODs, S/N = 3) were in the 
      range of 0.1 - 3.6 microg/kg. The method is simple, rapid, accurate and sensitive 
      enough for the analysis of HAAs in poultry products.
FAU - Shao, Bin
AU  - Shao B
AD  - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Peng, Zengqi
AU  - Peng Z
FAU - Yang, Hongsheng
AU  - Yang H
FAU - Wu, Guanghong
AU  - Wu G
FAU - Yao, Yao
AU  - Yao Y
FAU - Wan, Kehui
AU  - Wan K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amines)
RN  - 0 (Carcinogens)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Imidazoles)
RN  - 0 (Quinoxalines)
RN  - 77500-04-0 (2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline)
RN  - 909C6UN66T (2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine)
RN  - YRA7G7WU6P (3,4,8-trimethylimidazo(4,5-f)quinoxalin-2-amine)
SB  - IM
MH  - Amines/*analysis
MH  - Animals
MH  - Carcinogens/analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Analysis/methods
MH  - Food Contamination/*analysis
MH  - Heterocyclic Compounds/*analysis
MH  - Imidazoles/analysis
MH  - Poultry Products/*analysis
MH  - Quinoxalines/analysis
MH  - Solid Phase Extraction/methods
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):755-61.