PMID- 22128741
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Determination of propofol in human plasma by microemulsion liquid 
      chromatography].
PG  - 768-72
AB  - A microemulsion liquid chromatographic (MELC) method was developed for the 
      determination of propofol in human plasma. The assay was performed on a Hypersil 
      BDS C18 column, and the effects of each component in the microemulsion mobile 
      phase on the elution were investigated. The optimized microemulsion mobile phase 
      consisted of 0.5% acetic acid containing 3.0% sodium dodecylsulfate, 0.8% 
      n-heptane, 6.0% n-butanol. The flow rate was 1.0 mL/min, and the separation was 
      operated at ambient temperature. The fluorescence excitation and emission 
      wavelengths were 274 nm and 312 nm, respectively. The plasma samples were diluted 
      with the mobile phase and centrifuged before injection. The calibration curve of 
      propofol was linear within the range of 0.25 - 10 microg/mL. The method 
      recoveries were (98.2 +/- 1.9)% - (104.6 +/- 2.2)%. The intra-day relative 
      standard deviations (RSDs) of peak area were 1.42% -2.43%, and the inter-day RSDs 
      were 2.75% - 4.79%. The method is simple and reproducible. It can be used for the 
      determination of propofol in human plasma.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Department of Pharmacy, Xiangya Hospital of Central South University, Changsha 
      410008, China.
FAU - Cui, Ying
AU  - Cui Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anesthetics, Intravenous)
RN  - 0 (Emulsions)
RN  - YI7VU623SF (Propofol)
SB  - IM
MH  - Anesthetics, Intravenous/blood
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Emulsions
MH  - Humans
MH  - Propofol/*blood
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):768-72.