PMID- 22128744
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Preparation and evaluation of open tubular capillary electrochromatographic 
      column modified with carboxymethyl chitosan].
PG  - 786-90
AB  - Via covalent coupling of epoxy group, an open tubular column covalently modified 
      with carboxymethyl chitosan (CMC) as stationary phase was prepared. The 
      parameters of column pretreatment, silanization and chemically bonding of CMC 
      were optimized. The morphology of inner wall of the open tubular column was 
      observed by scanning electron microscope (SEM), which showed that the inner wall 
      of the prepared column was evenly coated with a polymer film. Either positive or 
      negative electroosmotic flow (EOF) could be achieved by varying the pH values of 
      running buffer. Good reproducibility was obtained with the relative standard 
      deviations (RSDs) of EOF less than 0.8% for run-to-run (n = 6), less than 3.5% 
      for day-to-day (n = 3), less than 4.3% for column-to-column (n = 3) and less than 
      6.1% for batch-to-batch (n = 3). Nucleotides (AMP, GMP, CMP and UMP) were 
      analyzed on the open tubular column modified with CMC, with high column 
      efficiencies ranging from 36 000 to 182 000 plates/m. The results indicate that 
      the developed method is effective, easy and stable.
FAU - Zhou, Sunying
AU  - Zhou S
AD  - Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 
      350108, China.
FAU - Lin, Xucong
AU  - Lin X
FAU - Xie, Zenghong
AU  - Xie Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nucleotides)
RN  - 0 (carboxymethyl-chitosan)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Capillary Electrochromatography/instrumentation/*methods
MH  - Chitosan/*analogs & derivatives/chemistry
MH  - Nucleotides/*analysis
MH  - Sensitivity and Specificity
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):786-90.