PMID- 22128745
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20201209
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 8
DP  - 2011 Aug
TI  - [Analysis of nine narcotics in urine by microemulsion electrokinetic 
      chromatography-field samplified sample injection].
PG  - 791-7
AB  - A simple, sensitive and reproducible method using microemulsion electrokinetic 
      chromatography (MEEKC)-field amplified sample injection (FASI) was developed for 
      the analysis of nine narcotics (morphine, codeine, naloxone, heroin, thebaine, 
      cocaine, pethidine, fentanyl and methadone) in urine. In the MEEKC method, sodium 
      dodecyl sulfate (SDS), 1-butanol and ethyl acetate were used as surfactant, 
      co-surfactant and organic solvent, respectively. The effects of the acidity and 
      concentration of borate buffer, SDS, 1-butanol and ethyl acetate contents were 
      investigated. The optimum concentrations (by mass fraction) of microemulsion 
      system were 0.6% SDS, 1.2% 1-butanol, 0.6% ethyl acetate and 97.6% 10 mmol/L 
      Na2B4O7 buffer (pH 9.5). The applied voltage was 25 kV. FASI was coupled with the 
      MEEKC method to increase the sensitivity. Under the optimum conditions, the nine 
      narcotics were baseline separated within 15 min and the detection limits (S/N = 
      3) were in the range of 0.3 - 8.0 microg/L. The spiked recoveries in urine 
      samples were between 79.4% and 119.9% with the intraday relative standard 
      deviations (RSDs) less than 5.5%. The developed method has been successfully 
      applied to the analysis of methadone in the samples from in vitro metabolism 
      study.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, 
      China.
FAU - Li, Qin
AU  - Li Q
FAU - Lu, Minghua
AU  - Lu M
FAU - Zhang, Lan
AU  - Zhang L
FAU - Chen, Guonan
AU  - Chen G
FAU - Cai, Zongwei
AU  - Cai Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Emulsions)
RN  - 0 (Narcotics)
RN  - 36B82AMQ7N (Naloxone)
RN  - 76I7G6D29C (Morphine)
RN  - UX6OWY2V7J (Codeine)
SB  - IM
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Codeine/urine
MH  - Emulsions
MH  - Humans
MH  - Morphine/urine
MH  - Naloxone/urine
MH  - Narcotics/*urine
EDAT- 2011/12/02 06:00
MHDA- 2012/09/07 06:00
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Aug;29(8):791-7.