PMID- 22129466
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20211021
IS  - 1742-481X (Electronic)
IS  - 1742-4801 (Print)
IS  - 1742-4801 (Linking)
VI  - 9
IP  - 4
DP  - 2012 Aug
TI  - Oxidant and antioxidant events during epidermal growth factor therapy to 
      cutaneous wound healing in rats.
PG  - 362-71
LID - 10.1111/j.1742-481X.2011.00895.x [doi]
AB  - Cutaneous wound healing is a highly complex process, which includes inflammation, 
      cell proliferation, matrix deposition and remodelling phases. Various growth 
      factors, like epidermal growth factor (EGF), play an important role during wound 
      healing. However, little is known about relationship between EGF and 
      oxidant-antioxidant events in cutaneous wound healing models. Thus we planned to 
      evaluate the connection between EGF therapy and oxidative stress in dermal tissue 
      followed by wounding. Fifty-four adult male Wistar-albino rats were randomly 
      divided into three groups: control, untreated and topical EGF administrated 
      group. A linear full-thickness excision of 40 mm in length on both sides of 
      spinal cord was made on the back of each rat and sutured under anaesthesia and 
      sterile conditions. Excision was closed with 4/0 atraumatic silk suture. EGF 
      solution was freshly prepared at 10 ng/ml dose in thilotears gel under aseptic 
      conditions. Following the surgery, 1 ml of EGF solution was administered to wound 
      strips one time in everyday. The animals were euthanised and wound tissues were 
      collected on days 1, 5, 7 and 14. Thiobarbituric acid reactive substans (TBARS), 
      glutathione (GSH), reactive nitrogen oxide species (NOx), ascorbic acid levels 
      and superoxide dismutase activity were measured spectrophotometrically. TBARS 
      levels decreased and NOx levels increased on day 5 after operation, and GSH 
      levels were increased on day 14 in EGF administered group compared with untreated 
      group. Our data showed that EGF may act like an antioxidant by scavenging toxic 
      oxidation products in wound tissue. In addition, it may contribute healing of the 
      wound tissue in earlier stages and suggest a potential effective role for 
      antioxidant therapies, especially until day 5.
CI  - (c) 2011 The Authors. (c) 2011 Blackwell Publishing Ltd and Medicalhelplines.com Inc.
FAU - Kalay, Zeynep
AU  - Kalay Z
AD  - Department of Biology, Faculty of Science, Gazi University, Ankara 06500, Turkey. 
      zeynepkalay85@gmail.com
FAU - Cevher, Sule Coskun
AU  - Cevher SC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111201
PL  - England
TA  - Int Wound J
JT  - International wound journal
JID - 101230907
RN  - 0 (Antioxidants)
RN  - 0 (Oxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Administration, Topical
MH  - Analysis of Variance
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Disease Models, Animal
MH  - Epidermal Growth Factor/*administration & dosage
MH  - Male
MH  - Nitric Oxide/metabolism
MH  - Oxidants/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Reference Values
MH  - Superoxide Dismutase/metabolism
MH  - Wound Healing/*drug effects
MH  - Wounds and Injuries/*drug therapy/metabolism
PMC - PMC7950618
EDAT- 2011/12/02 06:00
MHDA- 2012/12/12 06:00
PMCR- 2011/12/01
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - IWJ895 [pii]
AID - 10.1111/j.1742-481X.2011.00895.x [doi]
PST - ppublish
SO  - Int Wound J. 2012 Aug;9(4):362-71. doi: 10.1111/j.1742-481X.2011.00895.x. Epub 
      2011 Dec 1.