PMID- 22129603
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20211021
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 27
IP  - 1
DP  - 2012
TI  - Comparison of exoelectrogenic bacteria detected using two different methods: 
      U-tube microbial fuel cell and plating method.
PG  - 49-53
AB  - In a microbial fuel cell (MFC), exoelectrogens, which transfer electrons to the 
      electrode, have been regarded as a key factor for electricity generation. In this 
      study, U-tube MFC and plating methods were used to isolate exoelectrogens from 
      the anode of an MFC. Disparate microorganisms were identified depending on 
      isolation methods, despite the use of an identical source. Denaturing gel 
      gradient electrophoresis (DGGE) analysis showed that certain microorganisms 
      became dominant in the U-tube MFC. The predominant bacterium was similar to 
      Ochrobactrum sp., belonging to the Alphaproteobacteria, which was shown to be 
      able to function as an exoelectrogen in a previous study. Three isolates, one 
      affiliated with Bacillus sp. and two with Paenibacillus sp., were identified 
      using the plating method, which belonged to the Gram-positive bacteria, the 
      Firmicutes. The U-tube MFCs were inoculated with the three isolates using the 
      plating method, operated in the batch mode and the current was monitored. All of 
      the U-tube MFCs inoculated with each isolate after isolation from plates produced 
      lower current (peak current density: 3.6-16.3 mA/m(2)) than those in U-tube MFCs 
      with mixed culture (48.3-62.6 mA/m(2)). Although the isolates produced low 
      currents, various bacterial groups were found to be involved in current 
      production.
FAU - Yu, Jaecheul
AU  - Yu J
AD  - Department of Civil and Environmental Engineering, Pusan National University, 
      Busan, Korea.
FAU - Cho, Sunja
AU  - Cho S
FAU - Kim, Sunah
AU  - Kim S
FAU - Cho, Haein
AU  - Cho H
FAU - Lee, Taeho
AU  - Lee T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111201
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacteria/chemistry/classification/genetics/*isolation & purification
MH  - Bioelectric Energy Sources/*microbiology
MH  - Colony Count, Microbial/*methods
MH  - DNA, Bacterial/genetics
MH  - Electricity
MH  - Electrodes/microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
PMC - PMC4036030
EDAT- 2011/12/02 06:00
MHDA- 2012/09/11 06:00
PMCR- 2012/03/01
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - JST.JSTAGE/jsme2/ME11205 [pii]
AID - 27_49 [pii]
AID - 10.1264/jsme2.me11205 [doi]
PST - ppublish
SO  - Microbes Environ. 2012;27(1):49-53. doi: 10.1264/jsme2.me11205. Epub 2011 Dec 1.