PMID- 22131127
OWN - NLM
STAT- MEDLINE
DCOM- 20120817
LR  - 20211021
IS  - 1528-1132 (Electronic)
IS  - 0009-921X (Print)
IS  - 0009-921X (Linking)
VI  - 470
IP  - 7
DP  - 2012 Jul
TI  - Is monocyte chemotactic protein 1 elevated in aseptic loosening of TKA? A pilot 
      study.
PG  - 1879-84
LID - 10.1007/s11999-011-2191-5 [doi]
AB  - BACKGROUND: Failure of TKA from aseptic loosening is a growing concern, as TKA is 
      performed with increasing frequency. Loosening is multifactorial and may be 
      associated with elevated inflammatory cytokines in addition to biomechanical 
      failure. QUESTIONS/PURPOSES: We asked whether proinflammatory cytokines and 
      chemokines are elevated in synovial fluid from patients undergoing revision 
      surgery as compared to those with osteoarthritis (OA) or rheumatoid arthritis 
      (RA). METHODS: We obtained synovial fluid samples from 20 patients: six with 
      aseptic loosening of TKA (all with bone loss), 10 with primary OA, and four with 
      RA. A panel of cytokines/chemokines was screened using a SearchLight((R)) Array 
      (Pierce Biotechnology, Rockford, IL, USA) in one revision sample. Using these 
      data, we assayed the synovial fluids for monocyte chemotactic protein 1 (MCP-1) 
      by ELISA. RESULTS: We observed an increase in synovial MCP-1 levels in samples 
      from patients planned for TKA revision compared to those with OA or RA. In 
      patients undergoing revision arthroplasty, the mean (+/- SD) MCP-1 concentration 
      was 21,233 +/- 18,966 pg/mL (range, 1550-50,657 pg/mL; n = 6). In patients with OA, 
      the mean MCP-1 level was 3012 +/- 3321 pg/mL. In patients with RA, the mean MCP-1 
      concentration was 690 +/- 561 pg/mL. CONCLUSIONS: All patients undergoing revision 
      TKA showed elevated concentrations of MCP-1 compared to patients with OA and RA, 
      suggesting MCP-1 may serve as a potential marker or predictor of bone loss in 
      patients undergoing revision surgery. CLINICAL RELEVANCE: MCP-1 may be a novel 
      biomarker in patients showing early symptoms of aseptic loosening of TKA.
FAU - Dasa, Vinod
AU  - Dasa V
AD  - Department of Orthopaedics, Louisiana State University Health Sciences Center, 
      1542 Tulane Avenue, New Orleans, LA 70115, USA. vdasa@lsuhsc.edu
FAU - Kramer, Jill M
AU  - Kramer JM
FAU - Gaffen, Sarah L
AU  - Gaffen SL
FAU - Kirkwood, Keith L
AU  - Kirkwood KL
FAU - Mihalko, William M
AU  - Mihalko WM
LA  - eng
GR  - R01 DE018290/DE/NIDCR NIH HHS/United States
GR  - R01 AR054389/AR/NIAMS NIH HHS/United States
GR  - R03 DE014460/DE/NIDCR NIH HHS/United States
GR  - P20 RR017696/RR/NCRR NIH HHS/United States
GR  - F30 DE014831/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Clin Orthop Relat Res
JT  - Clinical orthopaedics and related research
JID - 0075674
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthritis, Rheumatoid/immunology/*surgery
MH  - Arthroplasty, Replacement, Knee/adverse effects/*instrumentation
MH  - Biomarkers/analysis
MH  - Chemokine CCL2/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Inflammation Mediators/*analysis
MH  - Male
MH  - Middle Aged
MH  - New York
MH  - Osteoarthritis, Knee/immunology/*surgery
MH  - Pilot Projects
MH  - *Prosthesis Failure
MH  - Reoperation
MH  - Synovial Fluid/*immunology
MH  - Treatment Failure
MH  - Up-Regulation
PMC - PMC3369079
EDAT- 2011/12/02 06:00
MHDA- 2012/08/18 06:00
PMCR- 2013/07/01
CRDT- 2011/12/02 06:00
PHST- 2011/12/02 06:00 [entrez]
PHST- 2011/12/02 06:00 [pubmed]
PHST- 2012/08/18 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - 2191 [pii]
AID - 10.1007/s11999-011-2191-5 [doi]
PST - ppublish
SO  - Clin Orthop Relat Res. 2012 Jul;470(7):1879-84. doi: 10.1007/s11999-011-2191-5.