PMID- 22132975
OWN - NLM
STAT- MEDLINE
DCOM- 20120321
LR  - 20211203
IS  - 1532-2335 (Electronic)
IS  - 1525-7770 (Linking)
VI  - 30
IP  - 12
DP  - 2011 Dec
TI  - Deoxyribose protects against rapamycin-induced cytotoxicity in colorectal cancer 
      cells in vitro.
PG  - 1197-202
LID - 10.1080/15257770.2011.602657 [doi]
AB  - Thymidine phosphorylase (TPase) is also known as the platelet-derived endothelial 
      cell growth factor (PD-ECGF) and plays a role in angiogenesis. Deoxyribose (dR; a 
      downstream TPase-product) addition to endothelial cells may stimulate FAK and 
      p70/S6k signaling, which can be inhibited by rapamycin. Rapamycin is a specific 
      mammalian target of the rapamycin (mTOR) inhibitor, a kinase that lies directly 
      upstream of p70/S6k. This suggests a role for TPase in the mTOR/p70/S6k pathway. 
      In order to study this in more detail, we exposed cells with and without TPase 
      expression to dR and rapamycin and determined the effect on cell growth. We 
      observed protection in cytotoxicity in Colo320 cells, but not Colo320 TP1 cells. 
      This was in part mediated by activation of p70/S6k and inhibition of autophagy. 
      Further studies are recommended to elucidate the mechanism behind the protective 
      effect of dR.
FAU - Bijnsdorp, I V
AU  - Bijnsdorp IV
AD  - Department of Urology, VU University Medical Center, Amsterdam, The Netherlands.
FAU - Peters, G J
AU  - Peters GJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Nucleosides Nucleotides Nucleic Acids
JT  - Nucleosides, nucleotides & nucleic acids
JID - 100892832
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 533-67-5 (Deoxyribose)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Autophagy/drug effects
MH  - Blotting, Western
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Colorectal Neoplasms/enzymology/*pathology
MH  - Cytoprotection/*drug effects
MH  - Deoxyribose/*pharmacology/therapeutic use
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Intracellular Space/drug effects/enzymology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Sirolimus/*toxicity
MH  - TOR Serine-Threonine Kinases/metabolism
EDAT- 2011/12/03 06:00
MHDA- 2012/03/22 06:00
CRDT- 2011/12/03 06:00
PHST- 2011/12/03 06:00 [entrez]
PHST- 2011/12/03 06:00 [pubmed]
PHST- 2012/03/22 06:00 [medline]
AID - 10.1080/15257770.2011.602657 [doi]
PST - ppublish
SO  - Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1197-202. doi: 
      10.1080/15257770.2011.602657.