PMID- 22134873
OWN - NLM
STAT- MEDLINE
DCOM- 20121011
LR  - 20191023
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 113
IP  - 4
DP  - 2012 Apr
TI  - MMP-3 provokes CTGF/CCN2 production independently of protease activity and 
      dependently on dynamin-related endocytosis, which contributes to human dental 
      pulp cell migration.
PG  - 1348-58
LID - 10.1002/jcb.24007 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) expression is promoted after pulpotomy, and 
      application of MMP-3 to dental pulp after pulpotomy accelerates angiogenesis and 
      hard tissue formation. However, the mechanism by which MMP-3 promotes dental pulp 
      wound healing is still unclear. Connective tissue growth factor/CCN family 2 
      (CTGF/CCN2), a protein belonging to the CCN family, is considered to participate 
      in wound healing, angiogenesis, and cell migration. In this study, we examined 
      the involvement of CTGF/CCN2 in MMP-3-induced cell migration in human dental pulp 
      (fibroblast-like) cells. In human dental pulp cells, MMP-3 promoted cell 
      migration, but this effect was clearly blocked in the presence of anti-CTGF/CCN2 
      antibody. MMP-3 provoked mRNA and protein expression and secretion of CTGF/CCN2 
      in a concentration- and time-dependent manner. The MMP-3 inhibitor NNGH failed to 
      suppress MMP-3-induced CTGF/CCN2 protein expression. The potent dynamin inhibitor 
      dynasore clearly inhibited MMP-3-induced CTGF/CCN2 expression. These results 
      strongly suggest that MMP-3 induces CTGF/CCN2 production independently of the 
      protease activity of MMP-3 and dependently on dynamin-related endocytosis, which 
      is involved in cell migration in human dental pulp cells.
CI  - (c) 2011 Wiley Periodicals, Inc.
FAU - Muromachi, Koichiro
AU  - Muromachi K
AD  - Department of Endodontics, Nihon University School of Dentistry at Matsudo, 
      Matsudo, Chiba 271-8587, Japan. mako08014@g.nihon-u.ac.jp
FAU - Kamio, Naoto
AU  - Kamio N
FAU - Narita, Takanori
AU  - Narita T
FAU - Annen-Kamio, Motoyo
AU  - Annen-Kamio M
FAU - Sugiya, Hiroshi
AU  - Sugiya H
FAU - Matsushima, Kiyoshi
AU  - Matsushima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (CCN2 protein, human)
RN  - 0 (DNA Primers)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Western
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Connective Tissue Growth Factor/*biosynthesis/genetics
MH  - DNA Primers
MH  - Dental Pulp/*cytology
MH  - Dynamins/antagonists & inhibitors/*physiology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Endocytosis/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Matrix Metalloproteinase Inhibitors
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/12/03 06:00
MHDA- 2012/10/12 06:00
CRDT- 2011/12/03 06:00
PHST- 2011/12/03 06:00 [entrez]
PHST- 2011/12/03 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - 10.1002/jcb.24007 [doi]
PST - ppublish
SO  - J Cell Biochem. 2012 Apr;113(4):1348-58. doi: 10.1002/jcb.24007.