PMID- 22137209
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20211021
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 145
IP  - 1-2
DP  - 2012 Jan 15
TI  - Proteomic alteration of Marc-145 cells and PAMs after infection by porcine 
      reproductive and respiratory syndrome virus.
PG  - 206-13
LID - 10.1016/j.vetimm.2011.11.005 [doi]
AB  - Viral infections usually result in alterations in the host cell proteome, which 
      determine the fate of infected cells and the progress of pathogenesis. To uncover 
      cellular protein responses in porcine reproductive and respiratory syndrome virus 
      (PRRSV), infected pulmonary alveolar macrophages (PAMs) and Marc-145 cells were 
      subjected to proteomic analysis involving two-dimensional electrophoresis (2-DE) 
      followed by MALDI-TOF-MS/MS identification. Altered expression of 44 protein 
      spots in infected cells was identified in 2D gels, of which the 29 characterised 
      by MALDI-TOF-MS/MS included 17 up-regulated and 12 down-regulated proteins. Some 
      of these proteins were further confirmed at the mRNA level using real-time 
      RT-PCR. Moreover, Western blot analysis confirmed the up-regulation of HSP27, 
      vimentin and the down-regulation of galectin-1. Our study is the first attempt to 
      analyze the cellular protein profile of PRRSV-infected Marc-145 cells using 
      proteomics to provide valuable information about the effects of PRRSV-induced 
      alterations on Marc-145 cell function. Further study of the affected proteins may 
      facilitate our understanding of the mechanisms of PRRSV infection and 
      pathogenesis.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Ding, Zhuang
AU  - Ding Z
AD  - College of Animal Science and Veterinary Medicine, Key Laboratory of Zoonosis, 
      Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, 
      PR China.
FAU - Li, Zhi-jie
AU  - Li ZJ
FAU - Zhang, Xiao-dong
AU  - Zhang XD
FAU - Li, Ya-gang
AU  - Li YG
FAU - Liu, Chang-jun
AU  - Liu CJ
FAU - Zhang, Yan-Ping
AU  - Zhang YP
FAU - Li, Yang
AU  - Li Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111117
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
SB  - IM
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Electrophoresis, Gel, Two-Dimensional/veterinary
MH  - Kidney/cytology/virology
MH  - Macrophages, Alveolar/chemistry/metabolism/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism
MH  - *Porcine respiratory and reproductive syndrome virus/metabolism
MH  - *Proteomics/methods
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary
MH  - Swine/virology
PMC - PMC7112595
EDAT- 2011/12/06 06:00
MHDA- 2012/07/24 06:00
PMCR- 2011/11/17
CRDT- 2011/12/06 06:00
PHST- 2010/12/21 00:00 [received]
PHST- 2011/11/09 00:00 [revised]
PHST- 2011/11/11 00:00 [accepted]
PHST- 2011/12/06 06:00 [entrez]
PHST- 2011/12/06 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
PHST- 2011/11/17 00:00 [pmc-release]
AID - S0165-2427(11)00450-8 [pii]
AID - 10.1016/j.vetimm.2011.11.005 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):206-13. doi: 
      10.1016/j.vetimm.2011.11.005. Epub 2011 Nov 17.