PMID- 221373
OWN - NLM
STAT- MEDLINE
DCOM- 19790816
LR  - 20140724
IP  - 24 Pt 2
DP  - 1978
TI  - Interferon in the replication of herpes simplex virus in normal and pathological 
      nerve cells.
PG  - 1055-66
AB  - Previous results obtained in experimental and clinical trials have demonstrated 
      that topical combined thrapy with human interferon (HI) and human colostral 
      secretory immunoglobulin A (S-IgA) is effective against herpetic corneal 
      infection. This therapy prevented encephalitis in rabbits but could not 
      completely prevent recurrences either in rabbits or in patients. A number of in 
      vitro studies were designed to elucidate the role of these factors in herpes 
      simplex virus replication in the nervous system, with the following results: (1) 
      HSV latency in trigeminal ganglia (TG) explanted from rabbits with experimental 
      herpetic keratitis, topically treated with HI or HI/S-IgA: HSV was recovered in 
      30% TG after 15-19 days co-cultivation on RK-13 cells. (2) HSV replication in 
      nervous ganglia and nerve of newborn rabbits in organ culture; influence of HI or 
      HI plus IgG: a restrictive HSV productive infection was demonstrated in this 
      system, although yields were always higher in nerve cultures. We were unable to 
      demonstrate a direct effect of HI on HSV-1 replication. When explants were 
      treated with HI and IgG before and after infection for 48 hours a delay in the 
      expression of HSV-1 was detected by co-cultivation. (3) Replication of HSV-1 and 
      HSV-2 in a C1300 murine neuroblastoma clone (NB41A3): both HSV types replicated 
      with titres of 10(3.4) for HSV-1 AND 10(4.8) for HSV-2 at 48 hours p.i.; CPE was 
      more marked for HSV-2 at 24 hours. HSV-specific antigens were demonstrated by the 
      immunoperoxidase technique.
FAU - de De La Pena, N C
AU  - de De La Pena NC
FAU - Bal, E
AU  - Bal E
FAU - Puricelli, L
AU  - Puricelli L
FAU - Diaz, A
AU  - Diaz A
FAU - De Lustig, E S
AU  - De Lustig ES
LA  - eng
PT  - Journal Article
PL  - France
TA  - IARC Sci Publ (1971)
JT  - IARC scientific publications
JID - 0364347
RN  - 0 (Immunoglobulin G)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Encephalitis/prevention & control
MH  - Ganglia, Spinal/microbiology
MH  - Humans
MH  - Immunoglobulin G/administration & dosage
MH  - Interferons/*pharmacology
MH  - Keratitis, Dendritic/*drug therapy/microbiology
MH  - Mice
MH  - Neoplasms, Experimental/microbiology
MH  - Neuroblastoma/microbiology
MH  - Neurons/*microbiology
MH  - Organ Culture Techniques
MH  - Rabbits
MH  - Simplexvirus/*drug effects
MH  - Trigeminal Ganglion/microbiology
MH  - Virus Replication/*drug effects
EDAT- 1978/01/01 00:00
MHDA- 1978/01/01 00:01
CRDT- 1978/01/01 00:00
PHST- 1978/01/01 00:00 [pubmed]
PHST- 1978/01/01 00:01 [medline]
PHST- 1978/01/01 00:00 [entrez]
PST - ppublish
SO  - IARC Sci Publ (1971). 1978;(24 Pt 2):1055-66.