PMID- 22137362
OWN - NLM
STAT- MEDLINE
DCOM- 20120924
LR  - 20131121
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 95
IP  - 3
DP  - 2012 Mar
TI  - Decrease in calcium-sensing receptor in the progress of diabetic cardiomyopathy.
PG  - 378-85
LID - 10.1016/j.diabres.2011.11.007 [doi]
AB  - To observe the dynamic expression of calcium-sensing receptor (CaSR) in 
      myocardium of diabetic rats and explore its role in diabetic cardiomyopathy 
      (DCM), 40 male Wistar rats were randomly divided into 4 groups including control, 
      diabetic-4 weeks, diabetic-8 weeks and spermine treatment groups (240 muM of 
      spermine in drinking water). The type 2 Diabetes mellitus (DM) models were 
      established by intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after 
      high-fat and high-sugar diet for one month. The echocardiographic parameters were 
      measured, cardiac morphology was observed by electron microscope and HE staining. 
      The intracellular calcium concentration ([Ca(2+)](i)) was detected by 
      laser-scanning confocal microscope. Western blot analyzed the expression of CaSR, 
      protein kinase C alpha(PKC-alpha) and calcium handling regulators, such as phospholamban 
      (PLN), Ca(2+)-ATPase (SERCA), and ryanodine receptor (RyR). Compared with control 
      group, [Ca(2+)](i) and the expression of CaSR, RyR and SERCA/PLN were decreased, 
      while PKC-alpha and PLN were significantly increased in a time-dependent manner in 
      diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure and 
      systolic and diastolic dysfunction, and spermine (CaSR agonist) could prevent or 
      slow its progression. These results indicate that the CaSR expression of 
      myocardium is reduced in the progress of DCM, and its potential mechanism is 
      related to the impaired intracellular calcium homeostasis.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Bai, Shu-zhi
AU  - Bai SZ
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
FAU - Sun, Jian
AU  - Sun J
FAU - Wu, Hao
AU  - Wu H
FAU - Zhang, Ning
AU  - Zhang N
FAU - Li, Hong-xia
AU  - Li HX
FAU - Li, Guang-wei
AU  - Li GW
FAU - Li, Hong-zhu
AU  - Li HZ
FAU - He, Wen
AU  - He W
FAU - Zhang, Wei-hua
AU  - Zhang WH
FAU - Zhao, Ya-jun
AU  - Zhao YJ
FAU - Wang, Li-na
AU  - Wang LN
FAU - Tian, Ye
AU  - Tian Y
FAU - Yang, Bao-feng
AU  - Yang BF
FAU - Yang, Guang-dong
AU  - Yang GD
FAU - Wu, Ling-yun
AU  - Wu LY
FAU - Wang, Rui
AU  - Wang R
FAU - Xu, Chang-qing
AU  - Xu CQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111203
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/analysis
MH  - Diabetes Mellitus, Experimental
MH  - Diabetic Cardiomyopathies/*metabolism
MH  - Homeostasis
MH  - Male
MH  - Myocardium/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Calcium-Sensing/analysis/*metabolism
MH  - Spermine/therapeutic use
EDAT- 2011/12/06 06:00
MHDA- 2012/09/25 06:00
CRDT- 2011/12/06 06:00
PHST- 2011/09/09 00:00 [received]
PHST- 2011/10/31 00:00 [revised]
PHST- 2011/11/07 00:00 [accepted]
PHST- 2011/12/06 06:00 [entrez]
PHST- 2011/12/06 06:00 [pubmed]
PHST- 2012/09/25 06:00 [medline]
AID - S0168-8227(11)00630-9 [pii]
AID - 10.1016/j.diabres.2011.11.007 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2012 Mar;95(3):378-85. doi: 
      10.1016/j.diabres.2011.11.007. Epub 2011 Dec 3.