PMID- 22138717
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
DP  - 2010
TI  - NANOSTRUCTURED PROBES FOR IN VIVO GENE DETECTION.
PG  - 143-165
AB  - The ability to visualize in real-time the expression dynamics and localization of 
      specific RNAs in vivo offers tremendous opportunities for biological and disease 
      studies including cancer detection. However, quantitative methods such as 
      real-time PCR and DNA microarrays rely on the use of cell lysates thus not able 
      to obtain important spatial and temporal information. Fluorescence proteins and 
      other reporter systems cannot image endogenous RNA in living cells. Fluorescence 
      in situ hybridization (FISH) assays require washing to achieve specificity, 
      therefore can only be used with fixed cells. Here we review the recent 
      development of nanostructured probes for living cell RNA detection, and discuss 
      the biological and engineering issues and challenges of quantifying gene 
      expression in vivo. In particular, we describe methods that use oligonucleotide 
      probes, combined with novel delivery strategies, to image the relative level, 
      localization and dynamics of RNA in live cells. Examples of detecting endogenous 
      mRNAs, as well as imaging their subcellular localization are given to illustrate 
      the biological applications, and issues in probe design, delivery and target 
      accessibility are discussed. The nanostructured probes promise to open new and 
      exciting opportunities in sensitive gene detection for a wide range of biological 
      and medical applications.
FAU - Bao, Gang
AU  - Bao G
FAU - Santangelo, Phillip
AU  - Santangelo P
FAU - Nitin, Nitin
AU  - Nitin N
FAU - Rhee, Won Jong
AU  - Rhee WJ
LA  - eng
GR  - PN2 EY018244/EY/NEI NIH HHS/United States
GR  - U01 HL080711-03/HL/NHLBI NIH HHS/United States
GR  - PN2 EY018244-03/EY/NEI NIH HHS/United States
GR  - U54 CA119338/CA/NCI NIH HHS/United States
GR  - U01 HL080711-04/HL/NHLBI NIH HHS/United States
GR  - PN2 EY018244-02/EY/NEI NIH HHS/United States
GR  - U01 HL080711/HL/NHLBI NIH HHS/United States
GR  - U54 CA119338-040002/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Nanomedicine (Chichester)
JT  - Nanomedicine : design and applications of magnetic nanomaterials, nanosensors, 
      and nanosystems
JID - 101480046
PMC - PMC3226718
MID - NIHMS132479
EDAT- 2010/01/01 00:00
MHDA- 2010/01/01 00:01
PMCR- 2011/11/30
CRDT- 2011/12/06 06:00
PHST- 2011/12/06 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2010/01/01 00:01 [medline]
PHST- 2011/11/30 00:00 [pmc-release]
AID - 10.1002/9783527628155.nanotech054 [doi]
PST - ppublish
SO  - Nanomedicine (Chichester). 2010:143-165. doi: 10.1002/9783527628155.nanotech054.