PMID- 22138984
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 78
IP  - 3
DP  - 2012 Feb
TI  - Microbial community composition and dynamics of moving bed biofilm reactor 
      systems treating municipal sewage.
PG  - 855-64
LID - 10.1128/AEM.06570-11 [doi]
AB  - Moving bed biofilm reactor (MBBR) systems are increasingly used for municipal and 
      industrial wastewater treatment, yet in contrast to activated sludge (AS) 
      systems, little is known about their constituent microbial communities. This 
      study investigated the community composition of two municipal MBBR wastewater 
      treatment plants (WWTPs) in Wellington, New Zealand. Monthly samples comprising 
      biofilm and suspended biomass were collected over a 12-month period. Bacterial 
      and archaeal community composition was determined using a full-cycle community 
      approach, including analysis of 16S rRNA gene libraries, fluorescence in situ 
      hybridization (FISH) and automated ribosomal intergenic spacer analysis (ARISA). 
      Differences in microbial community structure and abundance were observed between 
      the two WWTPs and between biofilm and suspended biomass. Biofilms from both 
      plants were dominated by Clostridia and sulfate-reducing members of the 
      Deltaproteobacteria (SRBs). FISH analyses indicated morphological differences in 
      the Deltaproteobacteria detected at the two plants and also revealed distinctive 
      clustering between SRBs and members of the Methanosarcinales, which were the only 
      Archaea detected and were present in low abundance (<5%). Biovolume estimates of 
      the SRBs were higher in biofilm samples from one of the WWTPs which receives both 
      domestic and industrial waste and is influenced by seawater infiltration. The 
      suspended communities from both plants were diverse and dominated by aerobic 
      members of the Gammaproteobacteria and Betaproteobacteria. This study represents 
      the first detailed analysis of microbial communities in full-scale MBBR systems 
      and indicates that this process selects for distinctive biofilm and planktonic 
      communities, both of which differ from those found in conventional AS systems.
FAU - Biswas, Kristi
AU  - Biswas K
AD  - The Centre for Microbial Innovation, School of Biological Sciences, The 
      University of Auckland, Auckland, New Zealand.
FAU - Turner, Susan J
AU  - Turner SJ
LA  - eng
PT  - Journal Article
DEP - 20111202
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Archaeal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Archaea/*classification/genetics
MH  - Bacteria/*classification/genetics
MH  - Biofilms/*growth & development
MH  - Bioreactors/*microbiology
MH  - *Biota
MH  - Cluster Analysis
MH  - DNA, Archaeal/chemistry/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genes, rRNA
MH  - In Situ Hybridization, Fluorescence
MH  - New Zealand
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Archaeal/genetics
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Sewage/*microbiology
PMC - PMC3264111
EDAT- 2011/12/06 06:00
MHDA- 2012/05/11 06:00
PMCR- 2012/08/01
CRDT- 2011/12/06 06:00
PHST- 2011/12/06 06:00 [entrez]
PHST- 2011/12/06 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - AEM.06570-11 [pii]
AID - 6570-11 [pii]
AID - 10.1128/AEM.06570-11 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2012 Feb;78(3):855-64. doi: 10.1128/AEM.06570-11. Epub 
      2011 Dec 2.