PMID- 22142730
OWN - NLM
STAT- MEDLINE
DCOM- 20120417
LR  - 20141120
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 49
IP  - 6-7
DP  - 2011 Dec 10
TI  - Covalent immobilization of catalase onto spacer-arm attached modified florisil: 
      characterization and application to batch and plug-flow type reactor systems.
PG  - 547-54
LID - 10.1016/j.enzmictec.2011.09.002 [doi]
AB  - Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and 
      glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations 
      of catalase onto modified supports were optimized to improve the efficiency of 
      the overall immobilization procedures. The V(max) values of catalase immobilized 
      via glutaraldehyde (CIG) and catalase immobilized via glutaraldehyde+6-amino 
      hexanoic acid (CIG-6-AHA) were about 0.6 and 3.4% of free catalase, respectively. 
      The usage of 6-AHA as a spacer arm caused about 40 folds increase in catalytic 
      efficiency of CIG-6-AHA (8.3 x 10(5) M(-)(1) s(-)(1)) as compared to that of CIG (2.1 x 10(4) 
      M(-)(1) s(-)(1)). CIG and CIG-6-AHA retained 67 and 35% of their initial activities at 5 
       degrees C and 71 and 18% of their initial activities, respectively at room temperature 
      at the end of 6 days. Operational stabilities of CIG and CIG-6-AHA were 
      investigated in batch and plug-flow type reactors. The highest total amount of 
      decomposed hydrogen peroxide (TAD-H(2)O(2)) was determined as 219.5 mumol for 
      CIG-6-AHA in plug-flow type reactor.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Alptekin, Ozlem
AU  - Alptekin O
AD  - Department of Chemistry, Faculty of Arts & Sciences, University of Cukurova, 
      01330 Adana, Turkey.
FAU - Tukel, S Seyhan
AU  - Tukel SS
FAU - Yildirim, Deniz
AU  - Yildirim D
FAU - Alagoz, Dilek
AU  - Alagoz D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110921
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Magnesium Silicates)
RN  - 1343-88-0 (Florisil)
RN  - EC 1.11.1.6 (Catalase)
RN  - T3C89M417N (Glutaral)
RN  - U6F3787206 (Aminocaproic Acid)
SB  - IM
MH  - Aminocaproic Acid/chemistry
MH  - Animals
MH  - *Bioreactors
MH  - *Catalase/metabolism
MH  - Cattle
MH  - Enzyme Stability
MH  - *Enzymes, Immobilized/metabolism
MH  - Glutaral/chemistry
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Magnesium Silicates/chemistry
MH  - Microscopy, Electron, Scanning
MH  - Surface Properties
EDAT- 2011/12/07 06:00
MHDA- 2012/04/18 06:00
CRDT- 2011/12/07 06:00
PHST- 2011/01/13 00:00 [received]
PHST- 2011/07/29 00:00 [revised]
PHST- 2011/09/09 00:00 [accepted]
PHST- 2011/12/07 06:00 [entrez]
PHST- 2011/12/07 06:00 [pubmed]
PHST- 2012/04/18 06:00 [medline]
AID - S0141-0229(11)00191-8 [pii]
AID - 10.1016/j.enzmictec.2011.09.002 [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2011 Dec 10;49(6-7):547-54. doi: 
      10.1016/j.enzmictec.2011.09.002. Epub 2011 Sep 21.