PMID- 22144443
OWN - NLM
STAT- MEDLINE
DCOM- 20120201
LR  - 20211021
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 85
IP  - 6
DP  - 2011 Dec
TI  - Evaluation of an IgY-based immunomagnetic enzyme-linked immunosorbent assay 
      system for detection of circulating Schistosoma japonicum antigen in serum 
      samples from patients in China.
PG  - 1054-9
LID - 10.4269/ajtmh.2011.11-0051 [doi]
AB  - We have developed a novel egg yolk antibody (IgY)-coated magnetic beads 
      antigen-capture immunoassay for detection of a circulating antigen of Schistosoma 
      japonicum in serum samples of patients in schistosomiasis-endemic areas of China. 
      This IgY-based immunomagnetic bead enzyme-linked immunosorbent assay 
      (IgY-IMB-ELISA) uses polyclonal IgY-coated magnetic beads as a capture antibody, 
      and a monoclonal IgG as a detection antibody. The sensitivity of the magnetic 
      immunoassay was 100% (40 of 40) in cases of acute infection and 91.5% (107 of 
      117) in chronic cases of schistosomiasis, and no positive reaction was found in 0 
      of 49 healthy persons. Cross-reactivity was 3.3% (1 of 33) with clonorchiasis and 
      0% (0 of 20) with paragonimiasis. There was a significant correlation between 
      ELISA absorbance value and egg count (eggs per gram feces) and a correlation 
      coefficient of 0.88 in a small sample of 14 patients. The results demonstrated 
      that the IgY-IMB-ELISA is a sensitive and specific assay for detection of human 
      schistosomiasis japonica.
FAU - Lei, Jia-hui
AU  - Lei JH
AD  - Department of Parasitology, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, People's Republic of China. leijiahui@hotmail.com
FAU - Su, Bing-tao
AU  - Su BT
FAU - Xu, Hong
AU  - Xu H
FAU - Shen, Ji-long
AU  - Shen JL
FAU - Guan, Xiao-hong
AU  - Guan XH
FAU - Feng, Zhen-qing
AU  - Feng ZQ
FAU - Li, Yong-long
AU  - Li YL
FAU - Xu, Ming-xing
AU  - Xu MX
FAU - Liu, Wen-qi
AU  - Liu WQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Antigens, Helminth)
RN  - 0 (IgY)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/*immunology
MH  - China
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Immunoglobulins/*immunology
MH  - Immunomagnetic Separation/*methods
MH  - Schistosoma japonicum/*immunology
MH  - Schistosomiasis japonica/*diagnosis/immunology/parasitology
MH  - Sensitivity and Specificity
PMC - PMC3225151
EDAT- 2011/12/07 06:00
MHDA- 2012/02/02 06:00
PMCR- 2012/12/01
CRDT- 2011/12/07 06:00
PHST- 2011/12/07 06:00 [entrez]
PHST- 2011/12/07 06:00 [pubmed]
PHST- 2012/02/02 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - 85/6/1054 [pii]
AID - 10.4269/ajtmh.2011.11-0051 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2011 Dec;85(6):1054-9. doi: 10.4269/ajtmh.2011.11-0051.