PMID- 22145672
OWN - NLM
STAT- MEDLINE
DCOM- 20120424
LR  - 20220414
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 13
IP  - 1
DP  - 2012 Jan 9
TI  - Biodegradable poly(vinyl alcohol)-polyethylenimine nanocomposites for enhanced 
      gene expression in vitro and in vivo.
PG  - 73-83
LID - 10.1021/bm201157f [doi]
AB  - Use of cationic polymers as nonviral gene vectors has several limitations such as 
      low transfection efficiency, high toxicity, and inactivation by serum. In this 
      study, varying amounts of low molecular weight branched polyethylenimine 1.8 kDa 
      (bPEI 1.8) were introduced on to a neutral polymer, poly(vinyl alcohol) (PVA), to 
      bring in cationic charge on the resulting PVA-PEI (PP) nanocomposites. We 
      rationalized that by introducing bPEI 1.8, buffering and condensation properties 
      of the proposed nanocomposites would result in improved gene transfer capability. 
      A series of PVA-PEI (PP) nanocomposites was synthesized using well-established 
      epoxide chemistry and characterized by IR and NMR. Particle size of the PP/DNA 
      complexes ranged between 120 to 135 nm, as determined by dynamic light scattering 
      (DLS), and DNA retardation assay revealed efficient binding capability of PP 
      nanocomposites to negatively charged nucleic acids. In vitro transfection of 
      PP/DNA complexes in HEK293, HeLa, and CHO cells revealed that the best working 
      formulation in the synthesized series, PP-3/DNA complex, displayed ~2-50-fold 
      higher transfection efficiency than bPEIs (1.8 and 25 kDa) and commercial 
      transfection reagents. More importantly, the PP/DNA complexes were stable over a 
      period of time, along with their superior transfection efficiency in the presence 
      of serum compared to serum-free conditions, retaining the nontoxic property of 
      low molecular weight bPEI. The in vivo administration of PP-3/DNA complex in 
      Balb/c mice showed maximum gene expression in their spleen. The study 
      demonstrates the potential of PP nanocomposites as promising nonviral gene 
      vectors for in vivo applications.
FAU - Goyal, Ritu
AU  - Goyal R
AD  - CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, 
      Delhi-110007, India.
FAU - Tripathi, Sushil K
AU  - Tripathi SK
FAU - Vazquez, Esther
AU  - Vazquez E
FAU - Kumar, Pradeep
AU  - Kumar P
FAU - Gupta, Kailash C
AU  - Gupta KC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111216
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - *DNA/chemistry/pharmacology
MH  - *Gene Expression
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors/chemistry/pharmacology
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanocomposites/*chemistry
MH  - *Polyethyleneimine/chemistry/pharmacology
MH  - *Polyvinyl Alcohol/chemistry/pharmacology
EDAT- 2011/12/08 06:00
MHDA- 2012/04/25 06:00
CRDT- 2011/12/08 06:00
PHST- 2011/12/08 06:00 [entrez]
PHST- 2011/12/08 06:00 [pubmed]
PHST- 2012/04/25 06:00 [medline]
AID - 10.1021/bm201157f [doi]
PST - ppublish
SO  - Biomacromolecules. 2012 Jan 9;13(1):73-83. doi: 10.1021/bm201157f. Epub 2011 Dec 
      16.