PMID- 22145773
OWN - NLM
STAT- MEDLINE
DCOM- 20120405
LR  - 20240109
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 14
IP  - 12
DP  - 2011 Dec
TI  - A polysaccharide isolated from Ecklonia cava fermented by Lactobacillus brevis 
      inhibits the inflammatory response by suppressing the activation of nuclear 
      factor-kappaB in lipopolysaccharide-induced RAW 264.7 macrophages.
PG  - 1546-53
LID - 10.1089/jmf.2010.1562 [doi]
AB  - We previously reported that the increment of carbohydrate content in the 
      Viscozyme((R)) L (Novozyme Corp., Oklahoma City, OK, USA) extract of Lactobacillus 
      brevis-fermented Ecklonia cava affected the inhibition of nitric oxide (NO) 
      production and that it might be related to the polysaccharide compound. However, 
      there is no report of anti-inflammatory effects of the polysaccharide or its 
      biological mechanism. Here, we investigated the anti-inflammatory effects of the 
      polysaccharide and its biological mechanism in lipopolysaccharide (LPS)-activated 
      RAW 264.7 cells. The polysaccharide isolated from the Viscozyme extract of L. 
      brevis-fermented E. cava (VLFEP) dose-dependently decreased LPS-stimulated NO 
      production without cytotoxicity. Also, VLFEP significantly decreased the 
      production of prostaglandin E(2) (PGE(2)) at the 100 mug/mL concentration. In 
      addition, VLFEP dose-dependently decreased the protein and mRNA expressions of 
      inducible NO synthase, whereas it slightly decreased those of cyclooxygenase 2 
      and only at the 100 mug/mL concentration. Moreover, VLEFP dose-dependently 
      decreased the productions and/or mRNA expressions of tumor necrosis factor-alpha and 
      interleukin-6, compared with those of LPS only-stimulated cells. In further 
      experiments, VLFEP considerably reduced the phosphorylation and degradation of 
      inhibitory kappaB as well as the translocation of nuclear transcription factor-kappaB 
      (NF-kappaB) p65 into the nucleus, and its DNA binding was markedly induced by LPS 
      stimulation. This study suggests that VLFEP exerts anti-inflammatory effects by 
      down-regulating the production and expression of pro-inflammatory cytokines and 
      mediators via inhibiting the NF-kappaB pathway in LPS-stimulated RAW 264.7 cells.
FAU - Lee, Won-Woo
AU  - Lee WW
AD  - Department of Marine Life Science, Jeju National University, Jeju, Korea.
FAU - Ahn, Ginnae
AU  - Ahn G
FAU - Arachchillage, Janaka Priyalal Wijesinghe
AU  - Arachchillage JP
FAU - Kim, Young Mog
AU  - Kim YM
FAU - Kim, Se-Kwon
AU  - Kim SK
FAU - Lee, Bae-Jin
AU  - Lee BJ
FAU - Jeon, You-Jin
AU  - Jeon YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
EIN - J Med Food. 2012 Feb;15(2):222
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cell Line
MH  - Cyclooxygenase 2/drug effects/genetics/metabolism
MH  - Down-Regulation/drug effects
MH  - Fermentation
MH  - Interleukin-6/antagonists & inhibitors/genetics/metabolism
MH  - Levilactobacillus brevis/*metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - NF-kappa B/*antagonists & inhibitors/genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/antagonists & inhibitors/genetics/metabolism
MH  - Phaeophyceae/*chemistry
MH  - Phosphorylation/drug effects
MH  - Polysaccharides/*isolation & purification/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/biosynthesis/genetics
EDAT- 2011/12/08 06:00
MHDA- 2012/04/06 06:00
CRDT- 2011/12/08 06:00
PHST- 2011/12/08 06:00 [entrez]
PHST- 2011/12/08 06:00 [pubmed]
PHST- 2012/04/06 06:00 [medline]
AID - 10.1089/jmf.2010.1562 [doi]
PST - ppublish
SO  - J Med Food. 2011 Dec;14(12):1546-53. doi: 10.1089/jmf.2010.1562.