PMID- 22147272
OWN - NLM
STAT- MEDLINE
DCOM- 20120420
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 402
IP  - 3
DP  - 2012 Jan
TI  - Rapid, simple, and highly sensitive analysis of drugs in biological samples using 
      thin-layer chromatography coupled with matrix-assisted laser 
      desorption/ionization mass spectrometry.
PG  - 1257-67
LID - 10.1007/s00216-011-5576-0 [doi]
AB  - Rapid and precise identification of toxic substances is necessary for urgent 
      diagnosis and treatment of poisoning cases and for establishing the cause of 
      death in postmortem examinations. However, identification of compounds in 
      biological samples using gas chromatography and liquid chromatography coupled 
      with mass spectrometry entails time-consuming and labor-intensive sample 
      preparations. In this study, we examined a simple preparation and highly 
      sensitive analysis of drugs in biological samples such as urine, plasma, and 
      organs using thin-layer chromatography coupled with matrix-assisted laser 
      desorption/ionization mass spectrometry (TLC/MALDI/MS). When the urine containing 
      3,4-methylenedioxymethamphetamine (MDMA) without sample dilution was spotted on a 
      thin-layer chromatography (TLC) plate and was analyzed by TLC/MALDI/MS, the 
      detection limit of the MDMA spot was 0.05 ng/spot. The value was the same as that 
      in aqueous solution spotted on a stainless steel plate. All the 11 psychotropic 
      compounds tested (MDMA, 4-hydroxy-3-methoxymethamphetamine, 
      3,4-methylenedioxyamphetamine, methamphetamine, p-hydroxymethamphetamine, 
      amphetamine, ketamine, caffeine, chlorpromazine, triazolam, and morphine) on a 
      TLC plate were detected at levels of 0.05-5 ng, and the type (layer thickness and 
      fluorescence) of TLC plate did not affect detection sensitivity. In addition, 
      when rat liver homogenate obtained after MDMA administration (10 mg/kg) was 
      spotted on a TLC plate, MDMA and its main metabolites were identified using 
      TLC/MALDI/MS, and the spots on a TLC plate were visualized by MALDI/imaging MS. 
      The total analytical time from spotting of intact biological samples to the 
      output of analytical results was within 30 min. TLC/MALDI/MS enabled rapid, 
      simple, and highly sensitive analysis of drugs from intact biological samples and 
      crude extracts. Accordingly, this method could be applied to rapid drug screening 
      and precise identification of toxic substances in poisoning cases and postmortem 
      examinations.
FAU - Kuwayama, Kenji
AU  - Kuwayama K
AD  - National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 
      277-0882, Japan. kuwayama@nrips.go.jp
FAU - Tsujikawa, Kenji
AU  - Tsujikawa K
FAU - Miyaguchi, Hajime
AU  - Miyaguchi H
FAU - Kanamori, Tatsuyuki
AU  - Kanamori T
FAU - Iwata, Yuko T
AU  - Iwata YT
FAU - Inoue, Hiroyuki
AU  - Inoue H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111207
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Psychotropic Drugs)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Chromatography, Thin Layer/economics/*methods
MH  - Humans
MH  - Liver/metabolism
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*blood/metabolism/*urine
MH  - Psychotropic Drugs/*blood/metabolism/*urine
MH  - Rats
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser 
      Desorption-Ionization/economics/*methods
MH  - Time Factors
EDAT- 2011/12/08 06:00
MHDA- 2012/04/21 06:00
CRDT- 2011/12/08 06:00
PHST- 2011/09/06 00:00 [received]
PHST- 2011/11/11 00:00 [accepted]
PHST- 2011/10/16 00:00 [revised]
PHST- 2011/12/08 06:00 [entrez]
PHST- 2011/12/08 06:00 [pubmed]
PHST- 2012/04/21 06:00 [medline]
AID - 10.1007/s00216-011-5576-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Jan;402(3):1257-67. doi: 10.1007/s00216-011-5576-0. Epub 
      2011 Dec 7.