PMID- 22148170
OWN - NLM
STAT- MEDLINE
DCOM- 20120515
LR  - 20211004
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 84
IP  - 2
DP  - 2012 Jan 17
TI  - Enhancing the coverage of the urinary metabolome by sheathless capillary 
      electrophoresis-mass spectrometry.
PG  - 885-92
LID - 10.1021/ac202407v [doi]
AB  - Sheathless capillary electrophoresis-mass spectrometry (CE-MS), using a porous 
      tip sprayer, is proposed for the first time for highly sensitive metabolic 
      profiling of human urine. A representative metabolite mixture and human urine 
      were used for evaluation of the sheathless CE-MS platform. For test compounds, 
      relative standard deviations (RSDs) for migration times and peak areas were below 
      2% and 12%, respectively, and an injection volume of only  approximately 8 nL resulted in 
      detection limits between 11 and 120 nM. Approximately 900 molecular features were 
      detected in human urine by sheathless CE-MS whereas about 300 molecular features 
      were found with classical sheath-liquid CE-MS. This difference can probably be 
      attributed to an improved ionization efficiency and increased sensitivity at low 
      flow-rate conditions. The integration of transient-isotachophoresis (t-ITP) as an 
      in-capillary preconcentration procedure in sheathless CE-MS further resulted in 
      subnanomolar limits of detection for compounds of the metabolite mixture, and 
      more than 1300 molecular features were observed in urine. Compared to the 
      classical CE-MS approaches, the integration of t-ITP combined with the use of a 
      sheathless interface provides up to 2 orders of magnitude sensitivity 
      improvement. Hence, sheathless CE-MS can be used for in-depth metabolic profiling 
      of biological samples, and we anticipate that this approach will yield unique 
      information in the field of metabolomics.
FAU - Ramautar, Rawi
AU  - Ramautar R
AD  - Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden 
      University Medical Center, Leiden, The Netherlands. R.Ramautar@lumc.nl
FAU - Busnel, Jean-Marc
AU  - Busnel JM
FAU - Deelder, Andre M
AU  - Deelder AM
FAU - Mayboroda, Oleg A
AU  - Mayboroda OA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111230
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/*urine
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Isotachophoresis
MH  - *Metabolome
MH  - *Metabolomics
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Urine/*chemistry
EDAT- 2011/12/14 06:00
MHDA- 2012/05/16 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/05/16 06:00 [medline]
AID - 10.1021/ac202407v [doi]
PST - ppublish
SO  - Anal Chem. 2012 Jan 17;84(2):885-92. doi: 10.1021/ac202407v. Epub 2011 Dec 30.