PMID- 22154215
OWN - NLM
STAT- MEDLINE
DCOM- 20120507
LR  - 20221207
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 414
DP  - 2012 Jan 1
TI  - Use of DGGE and COMPOCHIP for investigating bacterial communities of various 
      vermicomposts produced from different wastes under dissimilar conditions.
PG  - 664-71
LID - 10.1016/j.scitotenv.2011.11.045 [doi]
AB  - This study describes the use of denaturing gradient gel electrophoresis (DGGE) 
      and COMPOCHIP (i.e. a microarray targeting typical bacteria of stabilized organic 
      materials and pathogenic bacteria) for investigating the bacterial communities of 
      four different vermicomposts. These included a commercial vermicompost produced 
      from cattle manure (CM) and three vermicomposts produced at pilot-scale by 
      recycling: damaged tomato fruits (DT); olive-mill waste mixed with biosolids 
      (OB); and winery wastes (WW). DGGE provided distinctive fingerprints of each 
      vermicompost, which were statistically related to their particular chemical 
      features. The comparison of the various vermicompost fingerprints showed that 
      they contained bacterial communities with an average similarity coefficient of 
      close to 80%. COMPOCHIP detected the presence of Sphingobacterium, Streptomyces, 
      Alpha-Proteobacteria, Delta-Proteobacteria, and Firmicutes in all the 
      vermicomposts. COMPOCHIP showed differences in the abundance of particular 
      bacterial taxa among the vermicomposts, giving an idea about the usefulness of 
      each vermicompost in the search for bacteria valuable to biotechnology. The joint 
      use of DGGE and COMPOCHIP is a useful tool to compare vermicompost bacterial 
      communities and to assess the potential of different vermicomposts as bioactive 
      organic materials.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Fernandez-Gomez, Manuel J
AU  - Fernandez-Gomez MJ
AD  - Department of Environmental Protection, Estacion Experimental del Zaidin, 
      Profesor Albareda 1, 18008 Granada, Spain. manuelj.fernandez@eez.csic.es
FAU - Nogales, Rogelio
AU  - Nogales R
FAU - Insam, Heribert
AU  - Insam H
FAU - Romero, Esperanza
AU  - Romero E
FAU - Goberna, Marta
AU  - Goberna M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111207
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (DNA Primers)
RN  - 0 (Manure)
RN  - 0 (Olive Oil)
RN  - 0 (Plant Oils)
RN  - 0 (Soil)
RN  - 0 (Waste Products)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Bacteria/*genetics
MH  - Cattle
MH  - DNA Fingerprinting
MH  - DNA Primers/genetics
MH  - Denaturing Gradient Gel Electrophoresis/*methods
MH  - Electric Conductivity
MH  - Hydrogen-Ion Concentration
MH  - Solanum lycopersicum
MH  - Manure/analysis
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Olive Oil
MH  - Plant Oils
MH  - *Soil
MH  - *Soil Microbiology
MH  - Waste Products/analysis
EDAT- 2011/12/14 06:00
MHDA- 2012/05/09 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/08/11 00:00 [received]
PHST- 2011/10/20 00:00 [revised]
PHST- 2011/11/14 00:00 [accepted]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
AID - S0048-9697(11)01359-3 [pii]
AID - 10.1016/j.scitotenv.2011.11.045 [doi]
PST - ppublish
SO  - Sci Total Environ. 2012 Jan 1;414:664-71. doi: 10.1016/j.scitotenv.2011.11.045. 
      Epub 2011 Dec 7.