PMID- 22154615
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20200205
IS  - 1778-7254 (Electronic)
IS  - 1297-319X (Linking)
VI  - 79
IP  - 5
DP  - 2012 Oct
TI  - Adeno-associated virus-mediated osteoprotegerin gene transfer protects against 
      joint destruction in a collagen-induced arthritis rat model.
PG  - 482-7
LID - S1297-319X(11)00272-7 [pii]
LID - 10.1016/j.jbspin.2011.10.011 [doi]
AB  - OBJECTIVE: To evaluate the in vivo joint protection effect of recombinant 
      adeno-associated virus-mediated gene transfer of human osteoprotegerin 
      (rAAV-hOPG). METHODS: Collagen-induced arthritis (CIA) rat model was established. 
      CIA rats were randomly divided into three groups: CIA control group (PBS), 
      rAAV-EGFP (enhanced green fluorescent protein) group and rAAV-hOPG (100 muL/d) 
      group, which received corresponding intra-articular injection treatment. The 
      thickness of the palms and soles, arthritis index, radiological score, 
      pathological score, bone damage factor and protein expression of inflammatory 
      factors were measured and compared with normal control group rats. RESULTS: 
      Positive fluorescence of frozen section confirmed that rAAV-hOPG was efficiently 
      transduced into the synovial tissues of test rats. In rAAV-hOPG group compared 
      with CIA control group, the radiological score was 30.18% lower (P<0.05); the 
      expression of OPG protein was 93.41% higher (P<0.05); the expression of matrix 
      metalloproteinase-3 (MMP-3) protein was 35.38% lower (P<0.05); however, the 
      expression of IL-1beta was not significant; the scores of pannus and inflammation in 
      rAAV-hOPG group have no significant difference. CONCLUSION: These results suggest 
      that adeno-associated virus-mediated transfer of human osteoprotegerin is 
      effectively transducted into the synovial tissues of CIA model, and protects 
      against articular cartilage and bone destruction, but has no obvious efficiency 
      on inflammation. The results also demonstrate that gene transfer using rAAV-hOPG 
      may be a feasible and effective therapeutic candidate to treat or prevent joint 
      destruction in inflammatory arthritis.
CI  - Copyright (c) 2011. Published by Elsevier SAS.
FAU - Bao, Lizhi
AU  - Bao L
AD  - Rheumatology and Immunology Department, Changhai Hospital, the Second Military 
      Medical University, Shanghai 200433, China.
FAU - Zhu, Tingting
AU  - Zhu T
FAU - Zhao, Dongbao
AU  - Zhao D
FAU - Han, Xinhai
AU  - Han X
FAU - Guan, Jianlong
AU  - Guan J
FAU - Shi, Zhiqing
AU  - Shi Z
FAU - Zhang, Lanlin
AU  - Zhang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111209
PL  - France
TA  - Joint Bone Spine
JT  - Joint bone spine
JID - 100938016
RN  - 0 (Interleukin-1beta)
RN  - 0 (Osteoprotegerin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/metabolism/pathology/*prevention & control
MH  - Arthrography
MH  - Dependovirus/*genetics
MH  - Disease Models, Animal
MH  - Gene Transfer Techniques
MH  - Genetic Therapy/*methods
MH  - Interleukin-1beta/metabolism
MH  - Joints/metabolism/*pathology
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Osteoprotegerin/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2011/12/14 06:00
MHDA- 2013/07/03 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/06/07 00:00 [received]
PHST- 2011/10/03 00:00 [accepted]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - S1297-319X(11)00272-7 [pii]
AID - 10.1016/j.jbspin.2011.10.011 [doi]
PST - ppublish
SO  - Joint Bone Spine. 2012 Oct;79(5):482-7. doi: 10.1016/j.jbspin.2011.10.011. Epub 
      2011 Dec 9.