PMID- 22155427
OWN - NLM
STAT- MEDLINE
DCOM- 20120315
LR  - 20151119
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 106-107
DP  - 2012 Jan 15
TI  - Disruption of endocrine function in in vitro H295R cell-based and in in vivo 
      assay in zebrafish by 2,4-dichlorophenol.
PG  - 173-81
LID - 10.1016/j.aquatox.2011.11.006 [doi]
AB  - Chlorophenols in the aquatic environment have been of concern due to their 
      potential effects on human and wildlife. In the present study, the endocrine 
      disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and 
      in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were 
      used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure 
      to 0, 0.1, 0.3 or 1.0 mg 2,4-DCP/L resulted in less production of 17beta-estradiol 
      (E2) and alterations in transcript expressions of genes involved in 
      steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3betaHSD, 17betaHSD 
      and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3 mg 2,4-DCP/L on 
      concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) 
      were measured and expression of mRNA of selected genes in 
      hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of 
      zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by 
      down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP 
      resulted in greater concentrations of testosterone (T) and E2 along with greater 
      mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin 
      synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, 
      but up-regulated in males. The hepatic estrogenic receptor (ERalpha and ERbeta) and 
      vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. 
      The average number of eggs spawned was significantly less upon exposure to 
      2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of 
      hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription 
      of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and 
      disrupts steroidogenesis, which in turn, can cause adverse effects on 
      reproduction in fish.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Ma, Yanbo
AU  - Ma Y
AD  - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of 
      Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
FAU - Han, Jian
AU  - Han J
FAU - Guo, Yongyong
AU  - Guo Y
FAU - Lam, Paul K S
AU  - Lam PK
FAU - Wu, Rudolf S S
AU  - Wu RS
FAU - Giesy, John P
AU  - Giesy JP
FAU - Zhang, Xiaowei
AU  - Zhang X
FAU - Zhou, Bingsheng
AU  - Zhou B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111125
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Anthelmintics)
RN  - 0 (Biomarkers)
RN  - 0 (Chlorophenols)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Vitellogenins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3XMK78S47O (Testosterone)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Animals
MH  - Anthelmintics/toxicity
MH  - Biomarkers/metabolism
MH  - Cell Line, Tumor
MH  - Chlorophenols/*toxicity
MH  - Cytochrome P-450 Enzyme System/genetics/metabolism
MH  - Endocrine Disruptors/*toxicity
MH  - Endocrine System/*drug effects/metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Testosterone/genetics/metabolism
MH  - Vitellogenins/genetics/metabolism
MH  - Water Pollutants, Chemical/*toxicity
MH  - Zebrafish
EDAT- 2011/12/14 06:00
MHDA- 2012/03/16 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/08/22 00:00 [received]
PHST- 2011/11/12 00:00 [revised]
PHST- 2011/11/15 00:00 [accepted]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/03/16 06:00 [medline]
AID - S0166-445X(11)00322-5 [pii]
AID - 10.1016/j.aquatox.2011.11.006 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2012 Jan 15;106-107:173-81. doi: 10.1016/j.aquatox.2011.11.006. 
      Epub 2011 Nov 25.