PMID- 22155765
OWN - NLM
STAT- MEDLINE
DCOM- 20120627
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 19
IP  - 2
DP  - 2012 Feb
TI  - Development of a fluorescent microsphere immunoassay for detection of antibodies 
      against porcine reproductive and respiratory syndrome virus using oral fluid 
      samples as an alternative to serum-based assays.
PG  - 180-9
LID - 10.1128/CVI.05372-11 [doi]
AB  - For effective disease surveillance, rapid and sensitive assays are needed to 
      detect antibodies developed in response to porcine reproductive and respiratory 
      syndrome virus (PRRSV) infection. In this study, we developed a multiplexed 
      fluorescent microsphere immunoassay (FMIA) for detection of PRRSV-specific 
      antibodies in oral fluid and serum samples. Recombinant nucleocapsid protein (N) 
      and nonstructural protein 7 (nsp7) from both PRRSV genotypes (type I and type II) 
      were used as antigens and covalently coupled to Luminex fluorescent microspheres. 
      Based on an evaluation of 488 oral fluid samples with known serostatus, the oral 
      fluid-based FMIAs achieved >92% sensitivity and 91% specificity. For serum 
      samples (n = 1,639), the FMIAs reached >98% sensitivity and 95% specificity. The 
      assay was further employed to investigate the kinetics of the antibody response 
      in infected pigs. In oral fluid, the N protein was more sensitive for the 
      detection of early infection (7 and 14 days postinfection), but nsp7 detected a 
      higher level and longer duration of antibody response (28 days postinfection). In 
      serum, the antibodies specific to nsp7 and N proteins were detected as early as 7 
      days postinfection, and the responses lasted more than 202 days. This study 
      provides a framework from which a more robust assay could be developed to profile 
      the immune response to multiple PRRSV antigens in a single test. The development 
      of oral fluid-based diagnostic tests will change the way we survey diseases in 
      swine herds and improve our ability to cheaply and efficiently track PRRSV 
      infections in both populations and individual animals.
FAU - Langenhorst, Robert J
AU  - Langenhorst RJ
AD  - Department of Veterinary and Biomedical Science, South Dakota State University, 
      Brookings, South Dakota, USA.
FAU - Lawson, Steven
AU  - Lawson S
FAU - Kittawornrat, Apisit
AU  - Kittawornrat A
FAU - Zimmerman, Jeffrey J
AU  - Zimmerman JJ
FAU - Sun, Zhi
AU  - Sun Z
FAU - Li, Yanhua
AU  - Li Y
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Nelson, Eric A
AU  - Nelson EA
FAU - Fang, Ying
AU  - Fang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111207
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Viral)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/*isolation & purification
MH  - Cell Line
MH  - Fluorescence
MH  - Immunoassay/*methods
MH  - Microspheres
MH  - Nucleocapsid Proteins/genetics/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Recombinant Proteins/immunology
MH  - Saliva/immunology
MH  - Swine/immunology/virology
MH  - Viral Nonstructural Proteins/genetics/*immunology
PMC - PMC3272925
EDAT- 2011/12/14 06:00
MHDA- 2012/06/28 06:00
PMCR- 2012/08/01
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/06/28 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - CVI.05372-11 [pii]
AID - 05372-11 [pii]
AID - 10.1128/CVI.05372-11 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2012 Feb;19(2):180-9. doi: 10.1128/CVI.05372-11. Epub 2011 
      Dec 7.