PMID- 22156631
OWN - NLM
STAT- MEDLINE
DCOM- 20120821
LR  - 20120427
IS  - 1423-002X (Electronic)
IS  - 0378-7346 (Linking)
VI  - 73
IP  - 3
DP  - 2012
TI  - The immunoprotective role of interleukin-10 in abnormal pregnancy outcome induced 
      by Toxoplasma gondii infection.
PG  - 223-9
LID - 10.1159/000333310 [doi]
AB  - AIMS: To investigate the immunoprotective effect of IL-10 on pregnancy in 
      Toxoplasma gondii-infected C57BL/6 mice. METHODS: Twenty-four pregnant mice were 
      randomly divided into 3 equal groups, i.e. a control group (CG), an infected 
      group (IG) and an infected group treated with IL-10 (IPTG). Each IPTG and IG 
      mouse was infected with 400 T. gondii trophozoites on gestational day (GD) 8. 
      Each IPTG mouse was injected with 1 mug recombinant mouse IL-10 via a tail vein on 
      GD 10 and 12. The mice were sacrificed on day 7 postinfection. Qa-1 and NKG2A 
      were analyzed in the placenta by flow cytometry and real-time PCR. IL-4, IL-10 
      and IFN-gamma in the placenta supernatant were analyzed by ELISA. Statistical 
      analysis was performed by one-way ANOVA. RESULTS: IPTG mice showed a better 
      mental state, had larger fetuses and placentas with a better blood supply and a 
      lower resorption ratio compared to IG mice. NKG2A and Qa-1 were significantly 
      increased in IPTG mice compared to IG mice (p < 0.05). IL-4 and IL-10 in IPTG 
      placenta supernatant were increased (p < 0.05), but IFN-gamma was decreased (p < 
      0.05) compared to IG placenta supernatant. The ratios of IFN-gamma/IL-4 and 
      IFN-gamma/IL-10 were decreased in IPTG mice compared to IG mice (p < 0.05, p < 0.05). 
      CONCLUSION: IL-10 plays an immunoprotective role and improves the pregnancy 
      outcome of T. gondii-infected mice.
CI  - Copyright (c) 2011 S. Karger AG, Basel.
FAU - Zhang, Ruijin
AU  - Zhang R
AD  - Department of Immunology, Binzhou Medical College, 346 Guan-hai Road, Lai-shan, 
      Yantai, PR China.
FAU - Zhang, Haixia
AU  - Zhang H
FAU - Liu, Xianbing
AU  - Liu X
FAU - Fu, Qiang
AU  - Fu Q
FAU - Xu, Xiaoyan
AU  - Xu X
FAU - Hu, Xuemei
AU  - Hu X
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111207
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Klrc1 protein, mouse)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (Q surface antigens)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - DNA Primers/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Histocompatibility Antigens Class I/genetics/metabolism
MH  - Interleukin-10/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NK Cell Lectin-Like Receptor Subfamily C/genetics/metabolism
MH  - Pregnancy
MH  - *Pregnancy Complications, Parasitic
MH  - *Pregnancy Outcome
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Toxoplasma/pathogenicity
MH  - Toxoplasmosis/*immunology
EDAT- 2011/12/14 06:00
MHDA- 2012/08/22 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/05/05 00:00 [received]
PHST- 2011/09/16 00:00 [accepted]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/08/22 06:00 [medline]
AID - 000333310 [pii]
AID - 10.1159/000333310 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2012;73(3):223-9. doi: 10.1159/000333310. Epub 2011 Dec 7.