PMID- 22157622
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20211021
IS  - 1554-6578 (Electronic)
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 71
IP  - 1
DP  - 2012 Jan
TI  - The importance of 10q status in an outcomes-based comparison between 1p/19q 
      fluorescence in situ hybridization and polymerase chain reaction-based 
      microsatellite loss of heterozygosity analysis of oligodendrogliomas.
PG  - 73-82
LID - 10.1097/NEN.0b013e318240fa65 [doi]
AB  - 1p/19q codeletion is a favorable prognostic marker of oligodendrogliomas. 
      Although fluorescence in situ hybridization (FISH) and microsatellite-based 
      polymerase chain reaction (PCR) for loss of heterozygosity (LOH) are common 
      methods to test for 1p/19q codeletion, it is unclear which test is better at 
      prognostic stratification. This study analyzed outcomes of 111 oligodendrogliomas 
      with both 1p/19q FISH and LOH done at the time of diagnosis. Overall concordance 
      between the 2 assays was 81.1%. In grade III oligodendrogliomas, LOH was better 
      than FISH at survival stratification (p < 0.0001 for LOH vs p = 0.02 for FISH), 
      although increasing the stringency of FISH interpretation criteria improved 
      concordance and prognostic power. Oligodendrogliomas that were 1p/19q-codeleted 
      by FISH but also had 10q LOH were negative for 1p/19q codeletion by PCR analysis 
      in more than 70% of cases, with very poor survival in the grade III subset. Thus, 
      although PCR-based LOH is a better stratifier of 1p/19q status, FISH still has 
      clinical and prognostic utility, especially if 10q data can be incorporated.
FAU - Horbinski, Craig
AU  - Horbinski C
AD  - Department of Pathology, University of Kentucky, Lexington, Kentucky, USA. 
      craig.horbinski@uky.edu
FAU - Nikiforova, Marina N
AU  - Nikiforova MN
FAU - Hobbs, Jonathan
AU  - Hobbs J
FAU - Bortoluzzi, Stephanie
AU  - Bortoluzzi S
FAU - Cieply, Kathleen
AU  - Cieply K
FAU - Dacic, Sanja
AU  - Dacic S
FAU - Hamilton, Ronald L
AU  - Hamilton RL
LA  - eng
GR  - K08 CA155764/CA/NCI NIH HHS/United States
GR  - K08 CA155764-02/CA/NCI NIH HHS/United States
GR  - 1K08CA155764-01A1/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Cohort Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Loss of Heterozygosity/*genetics
MH  - Male
MH  - Microsatellite Repeats/*genetics
MH  - Middle Aged
MH  - Oligodendroglioma/*genetics/pathology
MH  - Polymerase Chain Reaction/methods
MH  - Prospective Studies
PMC - PMC3246063
MID - NIHMS343364
EDAT- 2011/12/14 06:00
MHDA- 2012/02/14 06:00
PMCR- 2013/01/01
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.1097/NEN.0b013e318240fa65 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2012 Jan;71(1):73-82. doi: 
      10.1097/NEN.0b013e318240fa65.