PMID- 22159099
OWN - NLM
STAT- MEDLINE
DCOM- 20120202
LR  - 20220409
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 31
IP  - 49
DP  - 2011 Dec 7
TI  - Early-life experience decreases drug-induced reinstatement of morphine CPP in 
      adulthood via microglial-specific epigenetic programming of anti-inflammatory 
      IL-10 expression.
PG  - 17835-47
LID - 10.1523/JNEUROSCI.3297-11.2011 [doi]
AB  - A critical component of drug addiction research involves identifying novel 
      biological mechanisms and environmental predictors of risk or resilience to drug 
      addiction and associated relapse. Increasing evidence suggests microglia and 
      astrocytes can profoundly affect the physiological and addictive properties of 
      drugs of abuse, including morphine. We report that glia within the rat nucleus 
      accumbens (NAcc) respond to morphine with an increase in cytokine/chemokine 
      expression, which predicts future reinstatement of morphine conditioned place 
      preference (CPP) following a priming dose of morphine. This glial response to 
      morphine is influenced by early-life experience. A neonatal handling paradigm 
      that increases the quantity and quality of maternal care significantly increases 
      baseline expression of the anti-inflammatory cytokine IL-10 within the NAcc, 
      attenuates morphine-induced glial activation, and prevents the subsequent 
      reinstatement of morphine CPP in adulthood. IL-10 expression within the NAcc and 
      reinstatement of CPP are negatively correlated, suggesting a protective role for 
      this specific cytokine against morphine-induced glial reactivity and drug-induced 
      reinstatement of morphine CPP. Neonatal handling programs the expression of IL-10 
      within the NAcc early in development, and this is maintained into adulthood via 
      decreased methylation of the IL-10 gene specifically within microglia. The effect 
      of neonatal handling is mimicked by pharmacological modulation of glia in 
      adulthood with ibudilast, which increases IL-10 expression, inhibits 
      morphine-induced glial activation within the NAcc, and prevents reinstatement of 
      morphine CPP. Taken together, we have identified a novel gene x early-life 
      environment interaction on morphine-induced glial activation and a specific role 
      for glial activation in drug-induced reinstatement of drug-seeking behavior.
FAU - Schwarz, Jaclyn M
AU  - Schwarz JM
AD  - Department of Psychology and Neuroscience, Duke University, Durham, North 
      Carolina 27705, USA. jaclyn.schwarz@duke.edu
FAU - Hutchinson, Mark R
AU  - Hutchinson MR
FAU - Bilbo, Staci D
AU  - Bilbo SD
LA  - eng
GR  - R01 DA025978-03/DA/NIDA NIH HHS/United States
GR  - F32DA030136/DA/NIDA NIH HHS/United States
GR  - R01DA025978/DA/NIDA NIH HHS/United States
GR  - R01 DA025978/DA/NIDA NIH HHS/United States
GR  - F32 DA030136-02/DA/NIDA NIH HHS/United States
GR  - F32 DA030136/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (CD11b Antigen)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Narcotics)
RN  - 130068-27-8 (Interleukin-10)
RN  - 36B82AMQ7N (Naloxone)
RN  - 76I7G6D29C (Morphine)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - CD11b Antigen/genetics/metabolism
MH  - Chromatography, Liquid
MH  - Conditioning, Operant/*drug effects
MH  - Corticosterone/blood
MH  - Drug-Seeking Behavior/drug effects/physiology
MH  - Epigenomics
MH  - Extinction, Psychological/drug effects
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Handling, Psychological
MH  - Immunoprecipitation
MH  - Interleukin-10/*blood/genetics
MH  - *Life Change Events
MH  - Male
MH  - Mass Spectrometry
MH  - Microglia/drug effects/*metabolism
MH  - Microinjections
MH  - Morphine/*pharmacology
MH  - Naloxone/pharmacology
MH  - Narcotic Antagonists/pharmacology
MH  - Narcotics/*pharmacology
MH  - Nucleus Accumbens/cytology/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reinforcement, Psychology
PMC - PMC3259856
MID - NIHMS347948
EDAT- 2011/12/14 06:00
MHDA- 2012/02/03 06:00
PMCR- 2012/06/07
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/02/03 06:00 [medline]
PHST- 2012/06/07 00:00 [pmc-release]
AID - 31/49/17835 [pii]
AID - 3742323 [pii]
AID - 10.1523/JNEUROSCI.3297-11.2011 [doi]
PST - ppublish
SO  - J Neurosci. 2011 Dec 7;31(49):17835-47. doi: 10.1523/JNEUROSCI.3297-11.2011.