PMID- 22159465
OWN - NLM
STAT- MEDLINE
DCOM- 20120420
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 402
IP  - 3
DP  - 2012 Jan
TI  - Metal-enhanced fluorescent probes based on silver nanoparticles and its 
      application in IgE detection.
PG  - 1057-63
LID - 10.1007/s00216-011-5591-1 [doi]
AB  - In this paper, a novel metal plasmon coupled with an aptamer-nucleotide 
      hybridized probe was fabricated and applied for protein detection. The specific 
      aptamer and single-strand oligonucleotide were chemically bound to silver 
      nanoparticles (AgNPs), and Cy5-labeled, complementary single-strand 
      oligonucleotides were hybridized with the particle-bound oligonucleotides. The 
      hybridized DNA duplexes were regarded as rigid rods that separated the 
      fluorophore Cy5 and the surface of AgNPs to reduce the competitive quenching. 
      Using a model system comprising human immunoglobulin E (IgE) as the analyte and 
      goat antihuman IgE as immobilized capture antibody on glass slides, we 
      demonstrate that the detection performance of the synthetic probe was superior to 
      the aptamer-based fluorescent probes. The results showed a good linear 
      correlation for human IgE in the range from 10 ng/ml to 6.25 mug/ml. The detection 
      limit obtained was 1 ng/ml, which was 50 times lower than that using Cy5 
      oligonucleotide/aptamer hybrid duplex (Probe2) due to the metal-enhanced 
      fluorescence effect. This new strategy opens the possibility for the preparation 
      of high-sensitivity detection probes based on metal nanoparticles.
FAU - Wei, Xia
AU  - Wei X
AD  - Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 
      210009, China.
FAU - Li, Hui
AU  - Li H
FAU - Li, Zhonghui
AU  - Li Z
FAU - Vuki, Maika
AU  - Vuki M
FAU - Fan, Yu
AU  - Fan Y
FAU - Zhong, Wenying
AU  - Zhong W
FAU - Xu, Danke
AU  - Xu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111208
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (cyanine dye 5)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Antibodies, Immobilized/chemistry/immunology
MH  - Aptamers, Nucleotide/chemistry
MH  - Carbocyanines/chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis/immunology
MH  - Limit of Detection
MH  - Nanoparticles/*chemistry
MH  - Nucleic Acid Hybridization
MH  - Silver/*chemistry
EDAT- 2011/12/14 06:00
MHDA- 2012/04/21 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/08/24 00:00 [received]
PHST- 2011/11/20 00:00 [accepted]
PHST- 2011/10/26 00:00 [revised]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/04/21 06:00 [medline]
AID - 10.1007/s00216-011-5591-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Jan;402(3):1057-63. doi: 10.1007/s00216-011-5591-1. Epub 
      2011 Dec 8.