PMID- 22159609
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20131121
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 93
IP  - 6
DP  - 2012 Mar
TI  - Identification of suitable ionic liquids for application in the enzymatic 
      hydrolysis of rutin by an automated screening.
PG  - 2301-8
LID - 10.1007/s00253-011-3749-6 [doi]
AB  - An automated method in milliliter scale was developed for the screening of 
      process parameters concerning the hydrolysis of the flavonoid rutin catalyzed by 
      the rhamnosidase activity of naringinase from Penicillium decumbens. Besides the 
      effect of additives such as ionic liquids and low molecular salts, the 
      productivity in a multiple phase system as well as the recyclability of the 
      enzyme in repetitive batches were studied. The hydrophobic ionic liquid (IL) 
      trihexyl(tetradecyl)phosphonium bis(trifluormethylsulfonyl)imide 
      [P(h(3))t][Tf(2)N] was identified to combine the most favorable characteristics 
      out of 23 investigated ILs with regard to enzyme compatibility, substrate 
      solubility and enzyme partition coefficient. Also, for the corresponding cations 
      1-ethyl-3-methylimidazolium [EMIM], 1-butyl-3-methylimidazolium [BMIM], 
      1-butyl-1-methylpyrrolidinium [BMPL] and 1-octyl-3-methylimidazolium [OMIM], the 
      entity with the [Tf(2)N] anion was best tolerated by the naringinase. With 
      increasing IL content, higher space time yields with up to 1.5 g/(L h) for 80% 
      (v/v) [P(h(3))t][Tf(2)N] were achieved. Enhanced specific enzyme activity was 
      observed in the presence of Ca(2+) ions. By addition of [P(h(3))t][Tf(2)N] and 
      calcium chloride, the reactive aqueous phase was successfully used in three 
      repetitive batches with full conversion.
FAU - Temme, H
AU  - Temme H
AD  - Process Engineering, Helmut-Schmidt-Universitat, Hamburg, Germany. 
      temme@hsu-hh.de
FAU - Dethloff, O
AU  - Dethloff O
FAU - Pitner, W-R
AU  - Pitner WR
FAU - Fischer, S
AU  - Fischer S
FAU - Scheurich, R
AU  - Scheurich R
FAU - Schulte, M
AU  - Schulte M
FAU - Niemeyer, B
AU  - Niemeyer B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111210
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Fungal Proteins)
RN  - 0 (Ionic Liquids)
RN  - 0 (Multienzyme Complexes)
RN  - 5G06TVY3R7 (Rutin)
RN  - EC 3.2.1.- (naringinase)
RN  - EC 3.2.1.21 (beta-Glucosidase)
SB  - IM
MH  - Biocatalysis
MH  - Enzyme Stability
MH  - Fungal Proteins/chemistry/*metabolism
MH  - Hydrolysis
MH  - Ionic Liquids/*chemistry
MH  - Multienzyme Complexes/chemistry/*metabolism
MH  - Penicillium/*enzymology
MH  - Rutin/*chemistry
MH  - beta-Glucosidase/chemistry/*metabolism
EDAT- 2011/12/14 06:00
MHDA- 2012/06/19 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/08/17 00:00 [received]
PHST- 2011/11/17 00:00 [accepted]
PHST- 2011/11/02 00:00 [revised]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
AID - 10.1007/s00253-011-3749-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2012 Mar;93(6):2301-8. doi: 10.1007/s00253-011-3749-6. 
      Epub 2011 Dec 10.