PMID- 22160327
OWN - NLM
STAT- MEDLINE
DCOM- 20120412
LR  - 20211021
IS  - 1875-8355 (Electronic)
IS  - 1572-3887 (Print)
IS  - 1572-3887 (Linking)
VI  - 31
IP  - 1
DP  - 2012 Jan
TI  - The adaptor-related protein complex 2, alpha 2 subunit (AP2alpha2) gene is a 
      peroxisome proliferator-activated receptor cardiac target gene.
PG  - 75-83
LID - 10.1007/s10930-011-9379-0 [doi]
AB  - A peroxisome proliferator-actived receptor (PPAR) response element (RE) in the 
      promoter region of the adaptor-related protein complex 2, alpha 2 subunit (AP2alpha2) 
      of mouse heart has been identified. The steroid hormone nuclear PPARs and the 
      retinoid X receptors (RXRs) are important transcriptional factors that regulate 
      gene expression, cell differentiation and lipid metabolism. They form homo- (RXR) 
      and hetero- (PPAR-RXR) dimers that bind DNA at various REs. The AP2alpha2 gene is 
      part of complex and process that transports lipids and proteins from the plasma 
      membrane to the endosomal system. A PPAR activator (Wy14643) and DMSO (vehicle) 
      was introduced into control and delta337T thyroid hormone receptor (TRbeta1) transgenic 
      mice. Heart tissue was extracted and AP2alpha2 gene expression was compared using 
      Affymetrix expression arrays and qRT PCR among four groups [control, control with 
      Wy14643, delta337T TRbeta1 and delta337T TRbeta1 with Wy14643]. The gene expression of AP2alpha2 in 
      the Wy14643 control and transgenic mouse groups was significantly up regulated 
      over the vehicle mouse groups in both the array (p < 0.01) and qRT PCR (p < 0.01) 
      studies. Duplex oligo DNAs containing the PPAR/RXR motif (AGGTCA/TCCAGT) from the 
      AP2alpha2 promoter were used in EMSA to verify binding of the PPAR and RXR receptors 
      to their REs. pGL4.0 [Luc] constructs of the AP2alpha2 promoter with and without the 
      PPAR/RXR motifs were co-transfected with mouse PPARalpha, beta or gamma1 into HepG2 cells 
      and used in lucerifase assays to verify gene activation. In conclusion our study 
      revealed that PPARalpha regulates the mouse cardiac AP2alpha2 gene in both the control 
      and transgenic mouse.
FAU - Buroker, Norman E
AU  - Buroker NE
AD  - Department of Cardiology, Seattle Children's Research Institute, Seattle, WA 
      98101, USA. nburoker@u.washington.edu
FAU - Huang, Jie-Yu
AU  - Huang JY
FAU - Barboza, Julia
AU  - Barboza J
FAU - Ledee, Dolena R
AU  - Ledee DR
FAU - Eastman, Rocky J Jr
AU  - Eastman RJ Jr
FAU - Reinecke, Hans
AU  - Reinecke H
FAU - Ning, Xue-Han
AU  - Ning XH
FAU - Bassuk, James A
AU  - Bassuk JA
FAU - Portman, Michael A
AU  - Portman MA
LA  - eng
GR  - R01 HL060666/HL/NHLBI NIH HHS/United States
GR  - R01-HL-60666/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Netherlands
TA  - Protein J
JT  - The protein journal
JID - 101212092
RN  - 0 (Adaptor Protein Complex 2)
RN  - 0 (Adaptor Protein Complex alpha Subunits)
RN  - 0 (PPAR alpha)
RN  - 0 (adaptor protein complex 2, alpha 2 subunit)
SB  - IM
MH  - Adaptor Protein Complex 2/*genetics/metabolism
MH  - Adaptor Protein Complex alpha Subunits/*genetics/metabolism
MH  - Animals
MH  - Cell Line
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - Myocardium/*metabolism
MH  - PPAR alpha/genetics/*metabolism
MH  - Promoter Regions, Genetic
MH  - Response Elements
MH  - *Up-Regulation
PMC - PMC3699174
MID - NIHMS480932
EDAT- 2011/12/14 06:00
MHDA- 2012/04/13 06:00
PMCR- 2013/07/02
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/04/13 06:00 [medline]
PHST- 2013/07/02 00:00 [pmc-release]
AID - 10.1007/s10930-011-9379-0 [doi]
PST - ppublish
SO  - Protein J. 2012 Jan;31(1):75-83. doi: 10.1007/s10930-011-9379-0.